{"product_id":"tribo-micrococcal-nuclease-tbs6020","title":"Tribo™ Micrococcal Nuclease","description":"\u003cp\u003eMicrococcal Nuclease is a recombinant non-specific endonuclease, which originally comes from Staphylococcus aureus. It is produced and purified from recombinant E. coli. The enzymatic protein is a monomer of 245 amino acids. The molecular weight is about 17kDa. This enzyme requires Ca\u003csup\u003e2+\u003c\/sup\u003e and is completely inactivated by EDTA or EGTA.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEliminate DNA\/RNA remnants in protein expression products. Under proper conditions, the nucleic acid level could be reduced to less than 10 pg\/mL.\u003c\/li\u003e\n\u003cli\u003eReduce the viscosity of the lysed host cell (e.g., E. coli), improving the recovery in the purification of bio-products.\u003c\/li\u003e\n\u003cli\u003eChromatin structure analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDegrade all forms of DNA and RNA (single-stranded, double-stranded, linear, and circular nucleic acids).\u003c\/li\u003e\n\u003cli\u003eCompletely digest nucleic acids to 5′-monophosphate-terminated oligonucleotides 2-5 bases in length.\u003c\/li\u003e\n\u003cli\u003eNo protease activity or viral contaminant.\u003c\/li\u003e\n\u003cli\u003ePerfect for a wide variety of applications where complete digestion of nucleic acids is desirable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eConcentration \u0026amp; Purity\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e10,000 U\/1mL — Purity \u0026gt;95%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage Buffer\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50% Glycerol\u003cbr\u003e\n50 mM Tris-HCl pH=8.0\u003cbr\u003e\n20 mM NaCl\u003cbr\u003e\n10 mM CaCl\u003csub\u003e2\u003c\/sub\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage Conditions\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe enzyme is stable in pH 7.0-9.5 for up to 2 years when stored at –20°C. DO NOT store at –70°C as freezing may cause the loss of activity.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS6020--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS6020-MNuclease-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS6020-MNuclease_MSDS-2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986489040975,"sku":"TBS6020","price":405.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/6020.png?v=1782812377","url":"https:\/\/www.danyel.co.il\/products\/tribo-micrococcal-nuclease-tbs6020","provider":"danyel biotech","version":"1.0","type":"link"}