{"product_id":"tribo-human-tgf-1-elisa-kit-tbs3085","title":"Tribo™ Human TGF-β1 ELISA Kit","description":"\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique (See Fig. 1). A monoclonal antibody specific for human TGF-β1 was pre-coated onto a microplate. Standards and samples are pipetted into the wells, and incubated with biotin-conjugated detection antibody (Detection A) specific for human TGF-β1. After incubation, the plate wells are aspirated without washing. Then, incubate with Streptavidin- HRP (Detection B). Following a wash to remove any unbound antibody and samples, an ultra-sensitive TMB substrate solution is added to the wells for color development. The color intensity is in proportion to the amount of TGF-β1 bound in the initial step.  The intensity of the color is measured by plate read at 450 nm.\u003c!--TBS3085--\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human TGF-β1 is typically 10 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human TGF-β1.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element \"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"wpb_single_image wpb_content_element vc_align_left\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cdiv class=\"vc_single_image-wrapper vc_box_border_grey\"\u003e\u003cimg class=\"vc_single_image-img lazyloaded\" title=\"TBS3235-0\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-0.png\" alt=\"TBS3235-0\" width=\"521\" height=\"106\" data-was-processed=\"true\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-29859\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3085_01.png\" alt=\"\" width=\"534\" height=\"288\" data-lazy-sizes=\"auto, (max-width: 534px) 100vw, 534px\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-29860\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3085_02.png\" alt=\"\" width=\"414\" height=\"340\" data-lazy-sizes=\"auto, (max-width: 414px) 100vw, 414px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1256\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"61\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"276\"\u003e\n\u003cstrong\u003eSTORAGE OF \u003c\/strong\u003e\u003cstrong\u003eOPENED\/ \u003c\/strong\u003e\u003cstrong\u003eRECONSTITUTED\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eHuman TGF-β1 Microplate\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3085A\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human TGF-β1.\u003c\/td\u003e\n\u003ctd width=\"276\"\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eHuman TGF-β1 Standard\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3085B\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e50 µl of Recombinant human TGF-β1 protein (100ng\/mL).\u003c\/td\u003e\n\u003ctd width=\"276\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3085C\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e2.1 ml of Biotin-Human TGF-β1 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"276\"\u003e \n\u003cp\u003eMay be stored for up to\u003c\/p\u003e\n\u003cp\u003e3 months at 2-8 °C.*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3085D\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e300 µl of Streptavidin-HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3085E\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e25 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eWash Buffer\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e12 ml of concentrated solution (10x)\u003cem\u003e.\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e12 ml of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"82\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"255\"\u003e6 ml of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003c\/p\u003e\n\u003cp\u003eThe kit contains sufficient materials to run an ELISA on one 96 well plate.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3085_Human_TGF-%CE%B21_ELISA_datasheet_2024.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3085_Human-TGF-%CE%B21_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986372681807,"sku":"TBS3085","price":1457.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3085-1.jpg?v=1782809728","url":"https:\/\/www.danyel.co.il\/products\/tribo-human-tgf-1-elisa-kit-tbs3085","provider":"danyel biotech","version":"1.0","type":"link"}