{"product_id":"tribo-bovine-qpcr-100-reactions-tbs42001-100","title":"Tribo™ Bovine QPCR, 100 reactions","description":"\u003cp\u003eThe Bovine QPCR Kit is designed for the target-DNA specific detection of bovine matter in food and animal feed ingredients. The assay uses a real-time PCR-based protocol with fluoresce-probe to detect target DNA. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for bovine (Fig.1-2, and Table 1). No cross reactivity is observed with other animal species (See Table 2). The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues, or health concerns.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHighly sensitive and specific for bovine derived DNA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh efficiency: \u003c\/strong\u003ethe optimal systemic conditions for PCR amplifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStreamlined protocol:\u003c\/strong\u003e just add DNA template and water.\u003c\/li\u003e\n\u003cli\u003eNo cross reactivity with other species\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage Conditions\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit is shipped on ice, and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003ePCR Protocol\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Set up PCR reaction for each sample in 25 µL\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-10012 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001-3.png\" alt=\"\" width=\"387\" height=\"153\"\u003e\u003c\/p\u003e\n\u003cp\u003eInternal control should be included as below: Positive Control (5 µL \/reaction), or Negative Control (5 µL\/reaction)\u003c\/p\u003e\n\u003cp\u003e2. Suggested PCR conditions\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-10013 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001-4.png\" alt=\"\" width=\"449\" height=\"125\" data-lazy-sizes=\"auto, (max-width: 449px) 100vw, 449px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 1: Bovine qPCR Amplification \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cimg class=\"alignnone wp-image-10014 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001-6.png\" alt=\"\" width=\"494\" height=\"330\" data-lazy-sizes=\"auto, (max-width: 494px) 100vw, 494px\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.2: DNA Concentration and qPCR amplification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-10015 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001-7.png\" alt=\"\" width=\"477\" height=\"307\" data-lazy-sizes=\"auto, (max-width: 477px) 100vw, 477px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable1. Bovine qPCR Sensitivity Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-10017 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001-8.png\" alt=\"\" width=\"481\" height=\"256\" data-lazy-sizes=\"auto, (max-width: 481px) 100vw, 481px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable2: Cross-reactivity Survey\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-10018 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001-9.png\" alt=\"\" width=\"602\" height=\"306\" data-lazy-sizes=\"auto, (max-width: 602px) 100vw, 602px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS42001--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001_Bovine_qPCR-kit_V03_2022.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS42001_CATTLE_QPCR_MSDS-2022.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"100 Tests","offer_id":45986984853583,"sku":"TBS42001-100","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"200 Tests","offer_id":45986984886351,"sku":"TBS42001-200","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS42001-2.jpg?v=1782819213","url":"https:\/\/www.danyel.co.il\/products\/tribo-bovine-qpcr-100-reactions-tbs42001-100","provider":"danyel biotech","version":"1.0","type":"link"}