{"product_id":"transformax-ec100d-electrocompetent-e-coli-mbtool-008","title":"TransforMax EC100D Electrocompetent E. coli","description":"\u003cp\u003eThe TransforMax™ EC100D™ pir + Electrocompetent E. coli and TransforMax™ EC100D™ pir-116 Electrocompetent E. coli each constitutively express the π protein (the pir gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγ ori). The cells are derived from Epicentre's TransforMax™ EC100™ Electrocompetent E. coli by P1 phage transduction with a strain containing the pir + or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker.\u003c\/p\u003e\u003cp\u003eThe TransforMax EC100D pir + cells will maintain R6Kγ ori containing plasmids at approximately 15 copies per cell, 1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell. 1\u003c\/p\u003e\u003cp\u003eBenefits\u003c\/p\u003e\u003cp\u003eAccepts large clones for unbiased propagation and stability of large rescue clones.\u003cbr\u003elacZΔM15 for blue\/white screening of recombinants.\u003cbr\u003eRestriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.\u003cbr\u003eEndonuclease minus ( endA1) to ensure high yields of DNA.\u003cbr\u003eRecombination minus ( recA1) for greater stability of large cloned inserts.\u003c\/p\u003e\u003cp\u003eGenotypes\u003c\/p\u003e\u003cp\u003eTransforMax EC100D pir + Electrocompetent E. coli:\u003cbr\u003eF - mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str R) nupG pir +(DHFR)\u003c\/p\u003e\u003cp\u003eTransforMax EC100D pir-116 Electrocompetent E. coli:\u003cbr\u003eF - mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str R) nupG pir-116(DHFR)\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003c\/p\u003e\u003cp\u003eGreater than 5 × 10 9 colony forming units (cfu)\/µg with pR6Kan control DNA.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eMetcalf, W.W. et al. (1994) Gene 138, 1.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRescue cloning of EZ-Tn5 transposed elements\u003c\/li\u003e\n\u003cli\u003ePropagate plasmids tagged with the EZ-Tn5 Insertion Kit\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEach product contains 5 tubes each containing 100 µL of electrocompetent cells, which is enough cells for 10 electroporations. A control vector containing and R6Kγori is also included with each product.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_MBTOOL-008_Tmax-EC100D-pir-cells\"\u003eTmax EC100D pir cells\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 Reactions","offer_id":45986440839247,"sku":"EC6P095H","price":2121.0,"currency_code":"ILS","in_stock":true},{"title":"10 Reactions (ECP09500)","offer_id":45986440872015,"sku":"ECP09500","price":2121.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TransforMax-EC100D-Electrocompetent-E-coli_a3cbe321-6eaa-4af8-901e-a6bc0492fa3e.jpg?v=1785956241","url":"https:\/\/www.danyel.co.il\/products\/transformax-ec100d-electrocompetent-e-coli-mbtool-008","provider":"danyel biotech","version":"1.0","type":"link"}