{"product_id":"transformax-ec100-competent-cells-comcel-011","title":"TransforMax EC100 Competent Cells","description":"\u003cp\u003eThe highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table. 1).\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eRoutine cloning of DNA up to 200 kb.\u003c\/p\u003e\u003cp\u003eBenefits\u003c\/p\u003e\u003cp\u003eHigh transformation efficiency with clones of all sizes, including BAC clones (Table. 1).\u003cbr\u003elacZΔM15 for blue\/white screening of recombinants.\u003cbr\u003eRestriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.\u003cbr\u003eEndonuclease minus (endA1) to ensure high yields of DNA.\u003cbr\u003eRecombination minus (recA1) for greater stability of large cloned inserts.\u003cbr\u003eDNA\tTransforMax EC100 Chemically Competent E. coli\tTransforMax EC100 Electrocompetent E. coli\u003cbr\u003epUC19\t1.4 × 108\t1.4 × 1010\u003cbr\u003e8.1-kb Clone\t1.3 × 107\tNot tested\u003cbr\u003e13.1-kb Clone\t4.3 × 106\t1.3 × 109\u003cbr\u003e23.1-kb Clone\t9.2 × 105\t3.0 × 108\u003cbr\u003e145-kb BAC Clone\tNot tested\t7 × 107\u003cbr\u003e13.1-kb clone directly from a ligation reaction\t2.2 × 105\t2.1 × 107\u003c\/p\u003e\u003cp\u003eTable. 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µL of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µL aliquot from a standard 10-µL ligation reaction. Results shown are in cfu\/µg of DNA and are the average transformation efficiencies obtained from several trials.\u003c\/p\u003e\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eF- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG\u003c\/p\u003e\u003cp\u003eTransforMax EC100 Electrocompetent E. coli\u003c\/p\u003e\u003cp\u003eTransformation efficiency of \u0026gt;1 × 1010 cfu\/µg of pUC19.\u003c\/p\u003e\u003cp\u003eTransforMax EC100 Chemically Competent E. coli\u003c\/p\u003e\u003cp\u003eTransformation efficiency of \u0026gt;5 × 108 cfu\/µg of pUC19.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGeneral cloning strain\u003c\/li\u003e\n\u003cli\u003eClone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.\u003c\/li\u003e\n\u003cli\u003eAchieve high efficiencies with convenient chemically competent format.\u003c\/li\u003e\n\u003cli\u003eIdeal for cloning, subcloning and plasmid preparation due to endA and rec mutations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eIncludes pUC19 control DNA.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-011_CC02810-TransforMax-EC100-Chemically-Competent-Ecoli\"\u003eCC02810 TransforMax EC100 Chemically Competent Ecoli\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-011_TMax-EC100-Chem-Comp-cells\"\u003eTMax EC100 Chem Comp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-011_TMax-EC100-Electrocomp-cells\"\u003eTMax EC100 Electrocomp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 Reactions","offer_id":45986439495759,"sku":"CC02810","price":888.0,"currency_code":"ILS","in_stock":true},{"title":"20 Reactions","offer_id":45986439528527,"sku":"EC10010","price":1936.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TransforMax-EC100-Competent-Cells_f0b0921a-97d2-47c1-95da-349accc6d595.jpg?v=1785956190","url":"https:\/\/www.danyel.co.il\/products\/transformax-ec100-competent-cells-comcel-011","provider":"danyel biotech","version":"1.0","type":"link"}