{"product_id":"total-antioxidant-capacity-tac-cuprac-colorimetric-assay-tbs2068-200","title":"Total Antioxidant Capacity (TAC)- CUPRAC Colorimetric Assay","description":"\u003cp\u003eOxidants, such as reactive oxygen species (ROS) can generate free radicals that can cause severe oxidative damage to cellular lipids, membranes, proteins, and DNA. Antioxidants can scavenge these free radicals and prevent cellular oxidative stress. Antioxidants are widely used as dietary supplements and in the industry as preservatives in food and cosmetics. Measurements of the total antioxidant capacity (TAC) are used as an integrated index to assess their ability to counteract oxidative stress-induced damage in cells.\u003c\/p\u003e\n\u003cp\u003eTribioScience’s Total Antioxidant Capacity Colorimetric Assay provides a quick, efficient, and sensitive method to measure TAC from cell lysate, plasma serum, urine, tissue homogenates, and food extracts. This assay is based on the reduction of Cu2+ to Cu+ by antioxidants like Trolox (Fig1). The resulting Cu+ specifically forms a colored complex with a colorimetric probe. The color intensity at 490nm is proportional to TAC in the sample.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAPPLICATION\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDirect Assays:\u003c\/strong\u003e serum, plasma, urine, tissues and other biological samples, food, and beverages.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery\/Pharmacology:\u003c\/strong\u003e Effects of drugs on TAC.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFEATURES\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitive and accurate:\u003c\/strong\u003e Sensitive and accurate. Use a 20 µL sample. Linear detection range from 15.63 to 1000 µM Trolox equivalents.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple and high throughput:\u003c\/strong\u003e The procedure involves the addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKIT CONTENTS FOR 200 ASSAYS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7127 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/03\/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20210326152618.png\" alt=\"\" width=\"512\" height=\"141\" data-lazy-sizes=\"auto, (max-width: 512px) 100vw, 512px\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSTORAGE CONDITIONS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at –20°C, protected from light. Shelf life: 6 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eFig. 1 The Reaction Principle\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cimg class=\"alignnone wp-image-7187 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/03\/unnamed-file.png\" alt=\"\" width=\"459\" height=\"154\" data-lazy-sizes=\"auto, (max-width: 459px) 100vw, 459px\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 2 Diagram for standard preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7186 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/03\/2068-2.png\" alt=\"\" width=\"601\" height=\"348\" data-lazy-sizes=\"auto, (max-width: 601px) 100vw, 601px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRELATED PRODUCTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/fast-hydrogen-peroxide-assay\/\"\u003e Amplex Red Hydrogen Peroxide Assay Kit (TBS2066)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/common-reagents\/hrp-fluoresce-substrate-system\/\"\u003e AmplexRed_HRP-System (TBS5026)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/tribo-tryptase-activity-assay\/\"\u003e Tryptase Activity Assay (TBS2101)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/%ce%b2-hexosaminidase-activity-assay\/\"\u003e β-Hexosaminidase Activity Assay (TBS2105)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/cytochrome-c-oxidase-activity-assay\/\"\u003e Cytochrome C Oxidase Activity Assay (TBS2115)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2068-200--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/03\/TBS2068-200_TAC-assay-V01_2021-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/03\/TBS2068-200_TAC-assay_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986445885519,"sku":"TBS2068-200","price":1776.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2068-1.jpg?v=1782811184","url":"https:\/\/www.danyel.co.il\/products\/total-antioxidant-capacity-tac-cuprac-colorimetric-assay-tbs2068-200","provider":"danyel biotech","version":"1.0","type":"link"}