{"product_id":"tissue-and-cells-genomicprep-mini-spin-kit-28904275","title":"Tissue and cells genomicPrep Mini Spin Kit","description":"\u003cp\u003eTissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from animal tissues and mammalian cell cultures, in just 90 min for tissues and 45 min for cells, using DNA spin columns.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRapid procedure produces high molecular weight gDNA with minimal shearing\u003c\/li\u003e\n\u003cli\u003eCapable of purifying up to 1.5 μg\/mg of tissue from an input tissue sample range of 5 to 50 mg, and up to 40 μg of genomic DNA per 5 x 10⁶ cells depending on cell type\u003c\/li\u003e\n\u003cli\u003eTotal DNA extraction time reduced significantly to 90 min\u003c\/li\u003e\n\u003cli\u003eProduces high-quality high molecular weight DNA and less degradation\u003c\/li\u003e\n\u003cli\u003eVerified in common downstream applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eHuman and animal genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.\u003c\/p\u003e\n\n\u003cp\u003eFor sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.\u003c\/p\u003e\n\n\u003cp\u003eTo minimize the incidence of DNA shearing during extraction, Tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells, including human genomic DNA.\u003c\/p\u003e\n\n\u003cp\u003eThe kit can be used for DNA isolation from diverse tissue types such as liver, kidney, and mouse tails with yields ranging from 0.5 to 1.5 μg of genomic DNA per mg of tissue. The kit consistently generates purified genomic DNA with optical density A260\/A280 readings of 1.8 or higher.\u003c\/p\u003e\n\n\u003cp\u003eThe gDNA produced can then be further analyzed in PCR, for SNP genotyping, and next generation sequencing (NGS) projects.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-51174-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"50","offer_id":45982271832143,"sku":"28904275","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"250","offer_id":45982271864911,"sku":"28904276","price":5367.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-8668-original-png_4eba0505-efc7-4e8b-9786-2ea562eb0b89.png?v=1785959700","url":"https:\/\/www.danyel.co.il\/products\/tissue-and-cells-genomicprep-mini-spin-kit-28904275","provider":"danyel biotech","version":"1.0","type":"link"}