{"product_id":"thrombin-protease-for-affinity-tag-removal-27084601","title":"Thrombin protease for affinity tag removal","description":"\u003cp\u003eThrombin is a serine protease purified from bovine plasma for digestion of GST-tagged proteins or other tagged proteins that contain the thrombin recognition sequence.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSpecific cleavage - enables site-specific cleavage of fusion proteins containing an accessible recognition sequence.\u003c\/li\u003e\n\u003cli\u003eFunctionally free of other clotting factors such as plasminogen and plasmin.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThrombin protease for fusion protein cleavage\u003c\/p\u003e\u003cp\u003eThrombin is used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin. The following vectors encode that sequence: pGEX-1λT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3.\u003c\/p\u003e\u003cp\u003eCytiva's thrombin protease is “protein friendly,” preserving protein structure and stability throughout purification to maximize samples and results. The product also streamlines methodology, allowing users to detect expressed proteins by direct labeling of fusion products in vitro.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eThrombin protease from Cytiva is used for GST cleavage in a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eDissociation of Endos from beads during study of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3154957\/\"\u003eprotein phosphatase 2A in mitosis\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurification of LR11 transmembrane and cytoplasmic domains during study of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3068303\/\"\u003ehuman APP binding protein LR11\/SorLA for NMR studies\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16077-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"500 units","offer_id":45982092886095,"sku":"27084601","price":884.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-6139-original-png_7b50364d-38b5-4299-85ca-eb3ac79c4025.png?v=1785958199","url":"https:\/\/www.danyel.co.il\/products\/thrombin-protease-for-affinity-tag-removal-27084601","provider":"danyel biotech","version":"1.0","type":"link"}