{"product_id":"tg1-electrocompetent-cells-comcel-014","title":"TG1 Electrocompetent Cells","description":"\u003cp\u003eTG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu\/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression\u003c\/p\u003e\u003cp\u003eFigure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.\u003c\/p\u003e\u003cp\u003eTable 1. Competent Cells for Phage Display libraries\u003c\/p\u003e\u003cp\u003eCell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003eTG1 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eSS320 (MC1061 F') Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eMC1061 F-Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eER2738 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePerfect for antibody phage display library creation\u003c\/li\u003e\n\u003cli\u003eThe highest efficiency TG1 competent cells available.\u003c\/li\u003e\n\u003cli\u003eCreate larger libraries; speed discovery\u003c\/li\u003e\n\u003cli\u003eBulk custom dispensing available with all of our phage display cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eTG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)\u003cbr\u003eSS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003cbr\u003eER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5\u003cbr\u003eMC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003etechnical note E.coli strains for phage display\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-014_60502-1-60502-2-TG1-Electrocompetent-Cells\"\u003e60502 1 60502 2 TG1 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-014_MC1061F-Electro\"\u003eMC1061F Electro\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-014_Phage-Display\"\u003ePhage Display\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-014_F302-US-phage-display-comp-cells\"\u003eF302 US phage display comp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_COMCEL-014_technical-note-E.coli-strains-for-phage-display\"\u003etechnical note E.coli strains for phage display\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986433499215,"sku":"60502-1","price":1317.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986433531983,"sku":"60502-2","price":2278.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TG1-Electrocompetent-Cells_beb4a083-a68d-4798-bc02-223062c0c9ae.jpg?v=1785956148","url":"https:\/\/www.danyel.co.il\/products\/tg1-electrocompetent-cells-comcel-014","provider":"danyel biotech","version":"1.0","type":"link"}