{"product_id":"tet-one-inducible-expression-system-634301","title":"Tet-One™ Inducible Expression System","description":"\u003cp\u003eThe Tet-One Inducible Expression System is a tetracycline-inducible gene expression system that allows you to establish a tightly inducible expression system for your gene of interest in mammalian cells using an all-in-one plasmid vector. The pTetOne Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation, and your gene of interest from the PTRE3GS promoter in the reverse orientation. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. In addition, the kit contains linear selection markers for resistance to puromycin and hygromycin, either of which can be cotransfected with the pTetOne Vector to obtain stable clones. It also includes Xfect Transfection Reagent and Tet System Approved FBS.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAll-in-one vector design provides all the power of Tet-On 3G technology in a single vector, making inducible expression as easy as constitutive expression\u003c\/li\u003e\n\u003cli\u003eLow background and high maximum induced expression\u003c\/li\u003e\n\u003cli\u003eHigh sensitivity to doxycycline: near maximum expression at 10 ng\/ml doxycycline\u003c\/li\u003e\n\u003cli\u003eInducible expression in a broad range of tissues, because the Tet-On 3G transactivator is expressed from the human PGK promoter\u003c\/li\u003e\n\u003cli\u003eHigh-titer lentivirus packaging system provided with Lenti-X Tet-One systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTightly controlled inducible expression in mammalian cells\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST0206\/ST0206-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Tet\/Tet-One%20Inducible%20Expression%20System%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634301-102913.pdf\"\u003eTet-One Inducible Expression System Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTetOne%20Vector%20Information.pdf\"\u003epTetOne Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTetOne.gb\"\u003epTetOne Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTetOne-Luc%20Control.gb\"\u003epTetOne-Luc Control Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003elinear selection markers for resistance to puromycin and hygromycin, either of which can be cotransfected with the pTetOne Vector to obtain stable clones\u003c\/p\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631101\u003c\/td\u003e\n\u003ctd\u003eTet System Approved FBS, US-Sourced\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631106\u003c\/td\u003e\n\u003ctd\u003eTet System Approved FBS\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"Each","offer_id":45986052997199,"sku":"634301","price":8757.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/634301-Tet-One-Inducible-Expression-System-product-600.jpg?v=1782802709","url":"https:\/\/www.danyel.co.il\/products\/tet-one-inducible-expression-system-634301","provider":"danyel biotech","version":"1.0","type":"link"}