{"product_id":"tet-on-3g-bidirectional-inducible-expression-system-with-mcherry-631338","title":"Tet-On® 3G Bidirectional Inducible Expression System (with mCherry)","description":"\u003cp\u003eThe Tet-On 3G Inducible Expression System (with mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-mCherry Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTet-One systems (listed elsewhere) provide a complete inducible expression system on a single vector.\u003c\/li\u003e\n\u003cli\u003eRetroviral and lentiviral formats include the PTRE3GV promoter, which is optimized for use in retroviral and lentiviral vectors. They are supplied with complete viral packaging systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eInducible expression in mammalian cells\u003cbr\u003eInducible expression in stem cells and hematopoietic cells (EF1-alpha promoter version)\u003cbr\u003eCo-inducible expression of two genes of interest (bicistronic version)\u003cbr\u003eCo-express a fluorescent protein (mCherry and ZsGreen1 versions)\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST0206\/ST0206-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Tet\/Tet-On%203G%20Inducible%20Expression%20Systems%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631165-102318.pdf\"\u003eTet-On 3G Inducible Expression System (with mCherry) Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTRE3G-mCherry%20Vector%20Map_042314.pdf\"\u003epTRE3G-mCherry Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTRE3G-mCherry.gb\"\u003epTRE3G-mCherry  vector sequence file in .gb format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTRE3G-mCherry%20Vector%20Information.pdf\"\u003epTRE3G-mCherry Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTRE3G-ZsGreen1%20Vector%20Information.pdf\"\u003epTRE3G-ZsGreen1 Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTRE3G-Luc%20Control%20Vector%20Information.pdf\"\u003epTRE3G-Luc Control Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTRE3G-Luc%20Control.gb\"\u003epTRE3G-Luc Control Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pCMV-Tet3G_Vector_Information_041714.pdf\"\u003epCMV-Tet3G Vector Information\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pCMV-Tet3G.gb\"\u003epCMV-Tet3G vector sequence file in .gb format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631168-102318.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631338-102318.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631101\u003c\/td\u003e\n\u003ctd\u003eTet System Approved FBS, US-Sourced\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631106\u003c\/td\u003e\n\u003ctd\u003eTet System Approved FBS\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"Each","offer_id":45986026160207,"sku":"631338","price":8038.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/631338-Tet-On-3G-Bidirectional-Inducible-Expression-System-with-mCherry-product-600.jpg?v=1785957439","url":"https:\/\/www.danyel.co.il\/products\/tet-on-3g-bidirectional-inducible-expression-system-with-mcherry-631338","provider":"danyel biotech","version":"1.0","type":"link"}