{"product_id":"templiphi-100-500-amplification-kits-25640010","title":"TempliPhi 100\/500 Amplification Kits","description":"\u003cp\u003eTempliPhi™, based on Phi29 DNA polymerase, is used to exponentially amplify single- or double-stranded circular DNA by rolling circle amplification (RCA).\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare templates for cycle sequencing, cloning, and transformation from circular DNA starting material efficiently, in 4 to 6 hours\u003c\/li\u003e\n\u003cli\u003eGenerate microgram quantities of template DNA from picogram amounts of circular DNA\u003c\/li\u003e\n\u003cli\u003eUse amplified DNA directly for cycle sequencing without purification\u003c\/li\u003e\n\u003cli\u003eAmplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA\u003c\/li\u003e\n\u003cli\u003eReduces time, labor, and quantity of consumables needed for sequencing template preparation\u003c\/li\u003e\n\u003cli\u003eAllows easy automation of workflow for efficient low- to medium-throughput small DNA vector preparation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eTrends in rolling circle amplification: Capabilities for manipulation, error free production and assembly of nucleic acids are advancing. \u003ca href=\"\/Solutions\/Genomics\/Knowledge-center\/Trends-in-rolling-circle-amplification\"\u003eFind out how TempliPhi™ amplification kits use isothermal rolling circle amplification (RCA), enabled by phi29 DNA polymerase, to support these advancements\u003c\/a\u003e.\u003c\/p\u003e\n\n\u003cp\u003eOne-tube, one-temperature format\u003c\/p\u003e\n\n\u003cp\u003eTempliPhi™ kits use a unique kind of circular DNA amplification to efficiently prepare micrograms of DNA from picogram circular DNA starting material. TempliPhi™ kits use isothermal rolling circle amplification (RCA) for the exponential amplification of circular DNA using bacteriophage Phi29 DNA polymerase. The amplification used is often called multiple strand displacement amplification, or MDA. Phi29 DNA polymerase is active at 30°C, and isothermal amplification is performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol allows amplification of 96 samples from bacterial colonies with less than 20 min of hands-on time.\u003c\/p\u003e\n\n\u003cp\u003eTwo different types of TempliPhi™ DNA Amplification Kits are available to meet different throughput requirements. TempliPhi™ DNA Amplification Kit for 2000 reactions, designed for automation, contains pre-mixed components and requires overnight incubation. TempliPhi™ 100 and TempliPhi™ 500 reaction kits contain separate, unmixed components for enhanced stability and preparations are ready in 4 to 6 hours.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/Jm7lXqNi4EaTNwKaQTFrwg-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"100 reactions","offer_id":45982217371727,"sku":"25640010","price":1923.0,"currency_code":"ILS","in_stock":true},{"title":"500 reactions","offer_id":45982217404495,"sku":"25640050","price":8536.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-47399-original-png_75647777-b841-49d2-82c6-a794591e14e0.png?v=1785959130","url":"https:\/\/www.danyel.co.il\/products\/templiphi-100-500-amplification-kits-25640010","provider":"danyel biotech","version":"1.0","type":"link"}