{"product_id":"takara-seeker-spatial-transcriptomics-kit","title":"Seeker Spatial Transcriptomics Kit","description":"\u003cp\u003eSeeker Spatial Transcriptomics Kits enable whole transcriptome, spatial mapping of fresh frozen tissues at industry-leading resolution. The assay is compatible with your favorite eukaryotic organism and requires no new specialized instrumentation or novel probe sets.\u003c\/p\u003e\n\u003cp\u003eThe workflow starts by placing a tissue section on the Seeker tile—a monolayer of tightly-packed, spatially-indexed beads that preserves spatial information. Polyadenylated mRNA is captured and hybridized on the spatially-indexed beads before reverse transcription. The indexed beads are then dissociated from the tile and the tissue digested, followed by second strand synthesis and cDNA amplification. Purified cDNA undergoes NGS library preparation and is sequenced. The Seeker bioinformatics pipeline processes FASTQ files generated from Seeker sequencing libraries into spatial gene expression maps in a single, efficient package.\u003c\/p\u003e\n\u003cp\u003eThe pipeline also provides quality control and preliminary cluster and spatial variable gene analyses in a simple .html report for initial data interpretation of the Seeker experiment. Gene-by-bead matrices and spatial coordinates from the pipeline are provided in data formats (.rds, .h5ad, and .mtx) that are compatible with many spatial transcriptomic and single-cell analysis packages published in R and python.\u003c\/p\u003e\n\u003cp\u003ePlease see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAchieve single-cell resolution: A tightly-packed monolayer of beads delivers a continuous view of your region of interest at 10 μm resolution\u003c\/li\u003e\n\u003cli\u003eEnjoy true unbiased discovery: Analysis is not limited by predetermined targets\u003c\/li\u003e\n\u003cli\u003eLeverage standard molecular biology techniques: No new specialized instrumentation or microscopy expertise needed\u003c\/li\u003e\n\u003cli\u003eEnable flexibility across organisms and tissues: Analyze any polyadenylated RNA across a wide array of organisms and tissue types\u003c\/li\u003e\n\u003cli\u003eUtilize just the size you need: Conveniently offered in 3 mm x 3 mm or 10 mm x 10 mm tile sizes without sacrificing resolution\u003c\/li\u003e\n\u003cli\u003eGet fast time to results: Multiple tissue samples can be prepared for sequencing in just one day\u003c\/li\u003e\n\u003cli\u003eStreamline your bioinformatics pipeline workflow: Single, efficient package for generation of spatial gene expression maps\u003c\/li\u003e\n\u003cli\u003eWhole-transcriptome spatial mapping: Perform whole-transcriptome spatial mapping with validated protocols (Species compatibility: Eukaryotes)\u003c\/li\u003e\n\u003cli\u003eT-cell receptor (TCR) profiling with whole-transcriptome spatial mapping: Perform whole-transcriptome spatial mapping and TCR profiling with a user-demonstrated protocol. Follow the Seeker TCR Library Preparation User Guide in addition to the appropriate Seeker bundle user manual. (Species compatibility: Human and mouse)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eWhole-transcriptome spatial mapping\u003c\/li\u003e\n\u003cli\u003eT-cell receptor (TCR) profiling with whole-transcriptome spatial mapping\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePlex\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eWhole transcriptome\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eResolution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecial capital equipment\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNone required\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFresh frozen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAnalysis software included\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker bioinformatics pipeline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 3x3 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTile size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3 mm x 3 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 10x10 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTile size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 mm x 10 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 3x3 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDuration\/hands-on time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e8 hr\/2.5 hr\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 10x10 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDuration\/hands-on time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e8 hr\/2.5 hr\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 3x3 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended sequencing depth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e400 M\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 10x10 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended sequencing depth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 3x3 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRequired components\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker 3x3 Bundle V1.1; Seeker Dual Index Primer Kit v2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker 10x10 kit\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRequired components\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker 10x10 Bundle; Seeker Dual Index Primer Kit v2; Thermal Cycler Adaptor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eSeeker protocols by application\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWhole-transcriptome spatial mapping\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker 3x3 Bundle V1.1 User Manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWhole-transcriptome spatial mapping\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker 10x10 Bundle V1.1 User Manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eT-cell receptor (TCR) profiling with whole-transcriptome spatial mapping\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker TCR Library Preparation User Guide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eT-cell receptor (TCR) profiling with whole-transcriptome spatial mapping\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker 3x3 Bundle V1.1 User Manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eT-cell receptor (TCR) profiling with whole-transcriptome spatial mapping\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSeeker 10x10 Bundle V1.1 User Manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/resourcedocument\/x351387\" target=\"_blank\" rel=\"noopener\"\u003eSeeker 3x3 Bundle V1.1 User Manual [protocol for: Whole-transcriptome spatial mapping; protocol for: T-cell receptor (TCR) profiling with whole-transcriptome spatial mapping]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/resourcedocument\/x351396\" target=\"_blank\" rel=\"noopener\"\u003eSeeker 10x10 Bundle V1.1 User Manual [protocol for: Whole-transcriptome spatial mapping; protocol for: T-cell receptor (TCR) profiling with whole-transcriptome spatial mapping]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/resourcedocument\/x351452\" target=\"_blank\" rel=\"noopener\"\u003eSeeker TCR Library Preparation User Guide [protocol for: T-cell receptor (TCR) profiling with whole-transcriptome spatial mapping]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Cloud\/Cloud%20Analysis%20of%20Seeker%20and%20Trekker%20Data%20User%20Manual.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCloud Analysis of Seeker and Trekker Data User Manual [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Seeker\/Seeker%20TCR%20Library%20Preparation%20User%20Guide.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeeker TCR Library Preparation User Guide [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Secondary\/Secondary%20Data%20Analysis%20Workflow%20User%20Guide.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSecondary Data Analysis Workflow User Guide [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Seeker%203x3%20Bundle%20V1\/Seeker%203x3%20Bundle%20V1.1%20User%20Manual.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeeker 3x3 Bundle V1.1 User Manual [Seeker® 3x3 Bundle V1.1; Seeker® Dual Indexing Primer Kit V2]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Seeker%2010x10%20Bundle%20V1\/Seeker%2010x10%20Bundle%20V1.1%20User%20Manual.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeeker 10x10 Bundle V1.1 User Manual [Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Trekker%20and%20Seeker%20Preserving%20and%20Sectioning%20Tissue%20Protocol\/Trekker%20and%20Seeker%20Preserving%20and%20Sectioning%20Tissue%20Protocol-At-A-Glance.pdf\" target=\"_blank\" rel=\"noopener\"\u003eTrekker and Seeker Preserving and Sectioning Tissue Protocol-At-A-Glance [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/B003\/B003-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eBead Wash Buffer SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/B007\/B007-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003ecDNA Amp Buffer SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/E006\/E006-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003ecDNA Amp Enzyme SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/P003\/P003-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003ecDNA Amp Primer Mix SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/JW001\/JW001-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCryoCube SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/N001A\/N001A-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003edNTP SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/B005\/B005-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eHyb Buffer SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/E001\/E001-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eRNase Inhibitor SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/E003\/E003-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eRT Enzyme SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/B006\/B006-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eRT\/SS Buffer SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SQ003\/SQ003-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeeker 3x3 Tile V1.1 SDS (EU English) [Seeker® 3x3 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/E007\/E007-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSS Enzyme SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/P002\/P002-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSS Primer SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/B004\/B004-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eTC Buffer SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/E002\/E002-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eTC Enzyme SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/B009\/B009-EGHS-EN.pdf\" target=\"_blank\" rel=\"noopener\"\u003eTE SDS (EU English) [Seeker® 3x3 Bundle V1.1; Seeker® 10x10 Bundle V1.1]\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/products\/next-generation-sequencing\/spatial-omics\/trekker-single-cell-spatial-mapping-kit\" target=\"_blank\" rel=\"noopener\"\u003eTrekker Single Cell Spatial Mapping Kit\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"Seeker® 3x3 Bundle V1.1 (SK004)","offer_id":46733016334415,"sku":"SK004","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Seeker® 10x10 Bundle V1.1 (SK003)","offer_id":46733016367183,"sku":"SK003","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Seeker® Dual Indexing Primer Kit V2 (K006)","offer_id":46733016399951,"sku":"K006","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Seeker® Reaction Chamber Adapter (TJ002)","offer_id":46733016432719,"sku":"TJ002","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/SK004-Seeker-3x3-Bundle-V1-point-1-product-600.jpg?v=1788947352","url":"https:\/\/www.danyel.co.il\/products\/takara-seeker-spatial-transcriptomics-kit","provider":"danyel biotech","version":"1.0","type":"link"}