{"product_id":"tagged-column-package-his-2-step-purification-protocol-29497630","title":"Tagged column package, His 2-step purification protocol","description":"\u003cp\u003eThe column package allows you to combine affinity with size exclusion chromatography to obtain highly pure his-tagged proteins.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eContains a HisTrap HP column 1 mL prepacked with Ni Sepharose High Performance (HP) affinity resin for preparative purification of his-tagged proteins.\u003c\/li\u003e\n\u003cli\u003eYou achieve final purity with HiPrep 16\/60 Sephacryl S-200 HR size exclusion chromatography column, which removes any remaining trace of impurities or closely related substances and imidazole.\u003c\/li\u003e\n\u003cli\u003eIncludes instructions with guidance and tips showing you how to get started.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eNickel ion chelating groups for efficient capture of your his-tagged proteins\u003c\/p\u003e\n\n\u003cp\u003eNi Sepharose HP affinity resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups. Ni Sepharose HP has a binding capacity that exceeds 40 mg\/mL, resulting in high protein recovery.\u003c\/p\u003e\n\n\u003cp\u003eHigh final protein purity through size exclusion chromatography\u003c\/p\u003e\n\n\u003cp\u003eThe size exclusion column is prepacked with Sephacryl S-200 HR chromatography resin, which has a broad fractionation range of M\u003csub\u003er\u003c\/sub\u003e 5 000 to ~ 250 000 for globular proteins. The high sample capacity of the HiPrep column lets you load samples of up to 5 mL.\u003c\/p\u003e\n\n\u003cp\u003e\u003ca href=\"\/Solutions\/Protein-Research\/Knowledge-center\/Protein-purification-methods\/How-to-combine-chromatography-techniques\/His-tagged-protein-purification-protocols\"\u003eFind out more about how to set up a purification protocol for his-tagged proteins.\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-36197-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 kit","offer_id":45982367973455,"sku":"29497630","price":4449.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-36168-original-png_1525f307-4d33-4cda-8238-dfd7604b84be.png?v=1785960665","url":"https:\/\/www.danyel.co.il\/products\/tagged-column-package-his-2-step-purification-protocol-29497630","provider":"danyel biotech","version":"1.0","type":"link"}