{"product_id":"t7-r-dna-polymerase-d7p9205k","title":"T7 R\u0026DNA Polymerase","description":"\u003cp\u003eT7 R\u0026amp;DNA™ Polymerase* is a recombinant mutant form of T7 RNA polymerase (Y639F mutant), in which Tyr-639 has been replaced by Phe. 1,2 This mutation enables T7 R\u0026amp;DNA Polymerase to incorporate 2´-deoxyribonucleoside triphosphates (dNTPs) into full-length transcripts much more efficiently than the corresponding wild-type enzyme, 1 while retaining the same catalytic activity for incorporation of canonical NTPs, and the same high promoter specificity as wild-type T7 RNA Polymerase. The ability of this mutant enzyme to incorporate certain 2´-modified-dNTPs (including but not limited to dNTPs having a 2´-fluorine or 2´-amino group) enables efficient in vitro synthesis of transcripts composed of mixed rNMP\/2´-dNMP or rNMP\/2´- modified-dNMP from any DNA template that is downstream of the T7 RNA polymerase promoter. Such modified transcripts are useful for many applications.\u003c\/p\u003e\u003cp\u003eNote: Complete substitution of one 2´-dNTP (or one 2´-modified dNTP) for a canonical rNTP in a T7 R\u0026amp;DNA Polymerase reaction results in a slight decrease in yield. Additional substitutions of 2´-dNTPs (or 2´-modified-dNTPs) for canonical rNTPs will further reduce yield of the transcript produced. Substitution with all four 2´-dNTPs (or 2´-modified dNTPs) will result in extremely low yields of transcript and is NOT RECOMMENDED.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eSousa, R. and Padilla, R. (1995) EMBO J. 14, 4609.\u003cbr\u003eU.S. Patent Nos. 5,849,546 and 6,107,037.\u003c\/p\u003e\u003cp\u003e*Covered by issued and\/or pending patents.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSynthesise \"RNA\" transcripts that also contain standard or modified dNTPs using this mutant T7 RNA polymerase\u003c\/li\u003e\n\u003cli\u003eGenerate \"RNA\" transcripts with both rNMP\/2'-dNMP or rNMP\/2'-modified-NMP composition\u003c\/li\u003e\n\u003cli\u003eCreate modified \"RNA\" transcripts that are resistant to RNase A-type RNases\u003c\/li\u003e\n\u003cli\u003eUse for a variety of applications where \"RNA\" with incorporated dNTPs is beneficial\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of T7 R\u0026amp;DNA Polymerase catalyses the incorporation of 1 nmol of ribonucleoside triphosphate into RNA in 1 hour at 37 °C under standard assay conditions using a DNA template with the T7 promoter.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003e5X Transcription Buffer: 0.2 M Tris-HCl (pH 7.5), 50 mM NaCl, 30 mM MgCl2, and 10 mM spermidine.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContents: T7 R\u0026amp;DNA Polymerase, 5X Reaction Buffer, 100 mM DTT.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_D7P9205K_T7-R-and-DNA-Polymerase\"\u003eT7 R and DNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_D7P9205K_T7-DNAP\"\u003eT7 DNAP\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_D7P9205K_T7-RNAP\"\u003eT7 RNAP\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_D7P9205K_T7-RNDNA-Polymerase\"\u003eT7 RNDNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_D7P9205K_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986439987279,"sku":"D7P9205K","price":4392.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/T7-R_2526DNA-Polymerase_43afa7de-c1af-42c1-9aab-eb46987c8afb.jpg?v=1785956214","url":"https:\/\/www.danyel.co.il\/products\/t7-r-dna-polymerase-d7p9205k","provider":"danyel biotech","version":"1.0","type":"link"}