{"product_id":"t-vector-pmd-20-3270","title":"T-Vector pMD™20","description":"\u003cp\u003eT-Vector pMD20 is a linearized vector with a single 3'-terminal thymidine at both ends. The T-overhang ends at the cloning site improves the efficiency of ligation of PCR products which contain A-overhangs at 3'-ends. T-Vector pMD20 is derived from a pUC19 backbone with modifications in the coding sequence to reduce the false positive rate and improve the positive colonies screened by blue\/white color on ampicillin\/IPTG\/X-Gal LB plates. In addition, an SP6 promoter has been inserted upstream of the multiple cloning site. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase (Cat. #2520). T-Vector pMD20 is supplied with a Control Insert (500 bp) DNA for positive control reactions.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eConcentration: 50 ng\/µl\u003c\/li\u003e\n\u003cli\u003eAmount: 1 µg\u003c\/li\u003e\n\u003cli\u003eVector size: pMD20 2,736 bp; pMD19 (Simple) 2,692 bp\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTA cloning\u003cbr\u003ePCR cloning\u003cbr\u003eSP6 expression (pMD20 only)\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3270\/3270.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0455\/SD0455-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3270\/3270_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/T-Vector%20pMD20%20%28Linear%29_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003eT-Vector pMD20 (Linear) Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 ug","offer_id":45985997848655,"sku":"3270","price":762.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3270-T-Vector-pMD20-product-600.jpg?v=1782801056","url":"https:\/\/www.danyel.co.il\/products\/t-vector-pmd-20-3270","provider":"danyel biotech","version":"1.0","type":"link"}