{"product_id":"superrox-reference-dye-pcramp-015","title":"SuperROX Reference Dye","description":"\u003cp\u003eSuperRox is a specially formulated version of the ROX (carboxy-X-rhodamine) fluorophore. This dye is commonly used as passive reference to normalise signal variations not related to the PCR amplification. Irregularities controlled with a ROX passive reference include inconsistency in pipetting, evaporation of solution, instability of baseline fluorescence, and laser or light source anomalies which all produce well-to-well variation.\u003c\/p\u003e\u003cp\u003eSuperROX is formulated for maximum water solubility so as to guard against “ROX drop”, a linear decrease in ROX signal occurring throughout the PCR reaction. Presumably, this drop is due to aggregation of hydrophobic ROX molecules into intermolecular homodimers, accompanied by “static quenching” of the fluorescent signal. SuperROX is an ancillary reagent that does not participate in the PCR reaction. The SuperROX signal remains consistent throughout the experiment thus providing a stable reference to correct the baseline fluorescence and normalise the amplified signal.\u003c\/p\u003e\u003cp\u003eSuperROX is for use only in machines requiring a passive reference dye and with optics capable of resolving the ROX fluorophore (Abs Max 585 nm \/ Em Max 606 nm). SuperROX is typically utilised at concentrations between 100 and 300 nanomolar in the final reaction mix. Further concentration optimisation may be necessary on certain model instrumentation.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_PCRAMP-015_%20SuperROX-concentration-15%C3%82%C2%B5M\"\u003eSuperROX concentration 15µM\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"concentration 15 µM, 1 mL","offer_id":45986473345103,"sku":"SR-1000-1","price":509.0,"currency_code":"ILS","in_stock":true},{"title":"concentration 15 µM, 10 mL","offer_id":45986473377871,"sku":"SR-1000-10","price":2452.0,"currency_code":"ILS","in_stock":true},{"title":"concentration 15 µM, 100 mL","offer_id":45986473410639,"sku":"SR-1000-100","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"concentration 15 µM, 1000 ml","offer_id":45986473443407,"sku":"SR-1000-1000","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/SuperROX-Reference-Dye_6d6c7b62-f77a-4c43-859b-1b08de74c9f9.jpg?v=1785956820","url":"https:\/\/www.danyel.co.il\/products\/superrox-reference-dye-pcramp-015","provider":"danyel biotech","version":"1.0","type":"link"}