{"product_id":"superoxide-dismutase-enzyme-activity-tbp0087","title":"Superoxide Dismutase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Soluble in distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e -20° C; -4° F\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 3000 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 95%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThat amount of enzyme which, under specified conditions of the assay, will cause a 50% inhibition in the rate of reduction of ferricytochrome C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe method of assay is based on the ability of superoxide dismutase to compete with ferricytochrome C for superoxide anions generated by the xanthine oxidase system. This results in inhibition of the rate of reduction of ferricytochrome C. The method of assay has been described by McCord and Fridovich.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSuperoxide dismutase (SOD) (EC 1.15.1.1) catalyzes the destruction (dismutation) of superoxide free radical ions as follows:\u003c\/p\u003e\n\u003cp\u003eThe superoxide (O2-) ion is believed to be responsible for lipid peroxidation and peroxidative hemolysis of erythrocytes. The action of superoxide dismutase, therefore, results in protection of the biological integrity of cells and tissues against the harmful effects of superoxide free radicals.\u003c\/p\u003e\n\u003cp\u003eSuperoxide dismutase is widely distributed in both plants and animals. It occurs in high concentrations in brain, liver, heart, erythrocytes and kidney. Three superoxide dismutases have been characterized according to their metal content. The enzyme from bovine and human erythrocytes contains copper and zinc, the one from chicken and rat liver mitochondria contains manganese while the enzyme from E. coli contains iron. Superoxide dismutase from bovine erythrocytes has a molecular weight of 32,500.\u003c\/p\u003e\n\u003cp\u003eOrgotein is the generic name, adopted by the United States Adopted Names (USAN) for Cu-Zn superoxide dismutases. Clinical trials indicate that orgotein has strong anti-inflammatory properties with almost no toxic side effects.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.05 M Potassium phosphate buffer, pH 7.8 containing 0.0001 M EDTA.\u003c\/li\u003e\n\u003cli\u003e0.0003 M Ferricytochrome C (3.9 mg\/ml) in buffer.\u003c\/li\u003e\n\u003cli\u003e0.0015 M Xanthine (0.25 mg\/ml) in buffer.\u003c\/li\u003e\n\u003cli\u003eXanthine oxidase solution (approximately 0.03-0.05 mg\/ml in buffer). Prepare fresh prior to assay.\u003c\/li\u003e\n\u003cli\u003eSuperoxide dismutase (SOD) in buffer. Dilute in buffer to yield a final concentration of 100-200 U\/ml. Must be prepared fresh immediately prior to assay.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 550 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following reagents:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003ePhosphate buffer 2.7 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eFerricytochrome C 0.1 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eXanthine 0.1 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eXanthine oxidase 0.1 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eIncubate in spectrophotometer at 25°C for 5 minutes and record the increase in absorbance at 550 nm for 3 minutes.\u003c\/p\u003e\n\u003col start=\"3\"\u003e\n\u003cli\u003eRepeat with several dilutions of Xanthine oxidase until a µE550 nm\/min of 0.025 is obtained. Use this dilution of Xanthine oxidase in the next step for the assay of Superoxide dismutase.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following reagents:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003ePhosphate buffer 2.6 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eFerricytochrome C 0.1 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eXanthine 0.1 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eXanthine oxidase (see Step 3) 0.1 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eTo the cuvette, add 0.1 ml of different dilutions of SOD until a µE550 nm\/min of 0.025 is obtained.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0087-10KU TBP0087-50KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0087-Superoxide-Dismutase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0087_Superoxide-Dismutase-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"10 KU","offer_id":45986501034063,"sku":"TBP0087-10KU","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"50 KU","offer_id":45986501066831,"sku":"TBP0087-50KU","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0087.png?v=1782812687","url":"https:\/\/www.danyel.co.il\/products\/superoxide-dismutase-enzyme-activity-tbp0087","provider":"danyel biotech","version":"1.0","type":"link"}