{"product_id":"superose-6-increase-small-scale-sec-columns-29091597","title":"Superose™ 6 Increase small-scale SEC columns","description":"\u003cp\u003eSuperose™ 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range M r ~ 5K to ~ 5M.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWide fractionation range: optimized to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments\u003c\/li\u003e\n\u003cli\u003eVery high resolution: small bead size and a narrow particle size distribution provide high resolution, for high protein purity\u003c\/li\u003e\n\u003cli\u003eHigh flow rates: rigid beads give excellent pressure\/flow properties\u003c\/li\u003e\n\u003cli\u003eEnhanced performance: improved resolution and runtime compared to predecessor\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e“Increase”: Cytiva's new generation of size exclusion chromatography columns\u003c\/p\u003e\n\n\u003cp\u003eSuperose™ 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose™ 6 Increase columns deliver higher resolution protein purification in shorter runtimes.\u003c\/p\u003e\n\n\u003cp\u003eFractionation of large proteins and protein complexes\u003c\/p\u003e\n\n\u003cp\u003eSuitable for both preparative and analytical applications, Superose™ 6 Increase SEC columns are the first choice for the characterization of large proteins and protein complexes. They can also be used the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.\u003c\/p\u003e\n\n\u003cp\u003eSuperose™ 6 and Superdex™ 200 analysis ranges are complementary\u003c\/p\u003e\n\n\u003cp\u003eSuperose™ 6 Increase is designed for separation of protein complexes and other very large molecules, including those with molecular weights exceeding M\u003csub\u003er\u003c\/sub\u003e \u003csup\u003e~\u003c\/sup\u003e400 000. The optimal fractionation range of Superdex™ 200 Increase is complementary, providing higher resolution for proteins with molecular weights below M\u003csub\u003er\u003c\/sub\u003e \u003csup\u003e~\u003c\/sup\u003e400 000.\u003c\/p\u003e\n\n\u003cp\u003eCleaning-in-place (CIP) capabilities\u003c\/p\u003e\n\n\u003cp\u003eThis agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.\u003c\/p\u003e\n\n\u003cp\u003eRegular cleaning can also increase column lifetime.\u003c\/p\u003e\n\n\u003cp\u003eChoose from 3 column sizes to suit varying application needs\u003c\/p\u003e\n\n\u003ctable class=\"table table-striped\"\u003e\n\t\u003cthead\u003e\n\t\u003c\/thead\u003e\n\t\u003ctbody\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eColumn\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eRecommended sample volume\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eRecommended operating flow\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eEfficiency (N\/m)\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eApplications \u003c\/strong\u003e\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eSuperose™ 6 Increase 3.2\/300\u003c\/td\u003e\n\t\t\t\u003ctd\u003e4 to 50 µL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e0.04 mL\/min\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u0026gt;48,000\u003c\/td\u003e\n\t\t\t\u003ctd\u003eSmall-scale preparative purification and analysis of large proteins and other biomolecules, when small sample and buffer consumption is important\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eSuperose™ 6 Increase 10\/300 GL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e25 to 500 µL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e0.5 mL\/min\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u0026gt; 48,000\u003c\/td\u003e\n\t\t\t\u003ctd\u003eStandard for small-scale preparative purification and analysis of large proteins and other biomolecules, especially protein complexes\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eSuperose™ 6 Increase 5\/150 GL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e4 to 50 µL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e0.3 mL\/min\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u0026gt; 42,000\u003c\/td\u003e\n\t\t\t\u003ctd\u003eRapid purity check and homogeneity analysis of large proteins and protein complexes\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003eFeedback on Superose™ 6 Increase SEC columns\u003c\/p\u003e\n\n\u003cp\u003e\"Now I can work on more samples, resulting in higher throughput. I can now purify a protein in 30 minutes instead of an hour.\" Dr. Tomohiro Nishizawa (Nureki Laboratory). Department of Biological Sciences, Graduate School of Science, The University of Tokyo\u003c\/p\u003e\n\n\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\n\n\u003cp\u003eResearchers use these pre-packed columns for a variety of applications, such as:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003eStudy of a \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26569474\/\" target=\"_blank\"\u003emagnetic sensing protein\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003eEvaluation of effects two proteins have on \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26414402\/\" target=\"_blank\"\u003emicrotubule nucleation\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003eProduction of \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26707548\/\" target=\"_blank\"\u003evirus-like particles\u003c\/a\u003e in \u003ci\u003eE. coli\u003c\/i\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17336-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17491-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"3.2 × 300 mm","offer_id":45982034919503,"sku":"29091598","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 x 150 mm","offer_id":45982034952271,"sku":"29091597","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"10 x 300","offer_id":45982034985039,"sku":"29091596","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7159-original-png_cb9eb806-93e3-4ff9-9542-b19add919f0a.png?v=1785957399","url":"https:\/\/www.danyel.co.il\/products\/superose-6-increase-small-scale-sec-columns-29091597","provider":"danyel biotech","version":"1.0","type":"link"}