{"product_id":"superdex-200-prep-grade-pg-sec-resin-in-hiload-columns-28989336","title":"Superdex™ 200 prep grade (pg) SEC resin in HiLoad™ columns","description":"\u003cp\u003eDesigned for high-resolution protein fractionation at preparative scales. Pre-packed with Superdex™ 200 pg resin, these size exclusion chromatography columns enable purification of mAbs, DNA fragments and other biomolecules with M r ~ 10 000 to ~ 600 000.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePreparative-grade: high purity and resolution\u003c\/li\u003e\n\u003cli\u003eTwo column sizes available: for samples up to 5 mL and up to 13 mL\u003c\/li\u003e\n\u003cli\u003ePrepacked columns: convenient and reproducible; can be used with standalone pump or chromatography system\u003c\/li\u003e\n\u003cli\u003eScalable SEC: resin properties and column dimensions optimized for preparative-scale protein purification; choose from two XK column sizes (16\/600 or 26\/600)\u003c\/li\u003e\n\u003cli\u003eCompatible with ÄKTA systems: fittings (1\/16”) for direct connection to ÄKTA systems and other chromatography systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eHiLoad Superdex 200 preparation grade resin is well suited for rapid, preparative protein purification and scale-up. Its selectivity curve gives a broad fractionation range from M\u003csub\u003er\u003c\/sub\u003e \u003csup\u003e~\u003c\/sup\u003e 10 000 to 600 000, with excellent resolving power for antibody monomers, dimers and contaminants of lower molecular weight, such as albumin and transferrin.\u003c\/p\u003e\n\n\u003cp\u003eHigh mechanical strength and hydrophilicity make this dextran-agarose composite resin suitable for larger scale applications that require high flow rates, minimal nonspecific interactions, and fast, effective cleaning-in-place (CIP) protocols.\u003c\/p\u003e\n\n\u003cp\u003eSuccessful use of Superdex 200 pg columns in research and process development\u003c\/p\u003e\n\n\u003cp\u003eSuperdex 200 prep grade columns are an excellent choice for many applications, including:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003eautomated \u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0021967315013461\" target=\"_blank\"\u003emonoclonal antibody production\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003eseparation of \u003ca href=\"https:\/\/www.jlr.org\/content\/47\/7\/1572.short\" target=\"_blank\"\u003eserum proteins\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003epurification of \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26380413\/\" target=\"_blank\"\u003ehuman papillomavirus 16 L2E7\u003c\/a\u003e fusion protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003eHow to scale up column chromatography to process larger sample volumes\u003c\/p\u003e\n\n\u003cp\u003eWhen scaling up size exclusion chromatography columns in order to process larger sample volumes in a single step, consider the following steps:\u003c\/p\u003e\n\n\u003col\u003e\n\t\u003cli\u003eOptimize high-resolution separation at small scale.\u003c\/li\u003e\n\t\u003cli\u003eMaintain the sample-to-column volume ratio and the sample concentration.\u003c\/li\u003e\n\t\u003cli\u003eIncrease the column volume by increasing the cross-sectional area (inner diameter) of the column.\u003c\/li\u003e\n\t\u003cli\u003eMaintain the bed height.\u003c\/li\u003e\n\t\u003cli\u003eRun the separation at the same linear flow rate as used on the smaller column.\u003c\/li\u003e\n\u003c\/ol\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003ctable class=\"table table-striped\"\u003e\n\t\u003cthead\u003e\n\t\u003c\/thead\u003e\n\t\u003ctbody\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eMaintain\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eIncrease\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eBed height\u003c\/td\u003e\n\t\t\t\u003ctd\u003eColumn diameter\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eLinear flow rate\u003c\/td\u003e\n\t\t\t\u003ctd\u003eVolumetric flow rate\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eSample composition\u003c\/td\u003e\n\t\t\t\u003ctd\u003eSample volume\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003eOther Cytiva size exclusion chromatography columns\u003c\/p\u003e\n\n\u003cp\u003eCytiva offers a comprehensive range of resins and pre-packed columns for size exclusion chromatography (a.k.a. gel filtration chromatography). In addition to Superdex 200 pg columns for preparative scale fractionations, include resins and pre-packed columns for:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003edesalting and buffer exchange\u003c\/li\u003e\n\t\u003cli\u003eanalytical column chromatography\u003c\/li\u003e\n\t\u003cli\u003esmall-scale purifications\u003c\/li\u003e\n\t\u003cli\u003efractionation across varying molecular weight ranges\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11217-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17491-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 120 mL","offer_id":45982028693583,"sku":"28989335","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 × 320 mL","offer_id":45982028726351,"sku":"28989336","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-4090-original-png_b2a8a122-5c4b-4f27-b3d1-7c6a15ba5dc6.png?v=1785957203","url":"https:\/\/www.danyel.co.il\/products\/superdex-200-prep-grade-pg-sec-resin-in-hiload-columns-28989336","provider":"danyel biotech","version":"1.0","type":"link"}