{"product_id":"superdex-200-increase-small-scale-sec-columns-28990944","title":"Superdex™ 200 Increase small-scale SEC columns","description":"\u003cp\u003eSuperdex™ 200 Increase size exclusion (also referred to as gel filtration) chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with M r ~ 10 000 to ~ 600 000.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEnhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.\u003c\/li\u003e\n\u003cli\u003eVery high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.\u003c\/li\u003e\n\u003cli\u003eHigh flow rates: rigid beads give excellent pressure\/flow properties.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e“Increase”: Cytiva's size exclusion chromatography columns\u003c\/p\u003e\n\n\u003cp\u003eSuperdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.\u003c\/p\u003e\n\n\u003cp\u003eA range of pre-packed columns is available to suit a variety of preparative and analytical applications (Table 1-1).\u003c\/p\u003e\n\n\u003cp\u003eSelectivity for small-scale purification and analysis of monoclonal antibodies\u003c\/p\u003e\n\n\u003cp\u003eSuperdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range M\u003csub\u003er\u003c\/sub\u003e \u003csup\u003e~\u003c\/sup\u003e 100 000 to \u003csup\u003e~\u003c\/sup\u003e 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations.\u003c\/p\u003e\n\n\u003cp\u003eThe outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.\u003c\/p\u003e\n\n\u003cp\u003eSuperdex™ 200 and Superose™ 6 analysis ranges are complementary\u003c\/p\u003e\n\n\u003cp\u003eSuperdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range M\u003csub\u003er\u003c\/sub\u003e \u003csup\u003e~\u003c\/sup\u003e 100 000 to \u003csup\u003e~\u003c\/sup\u003e 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.\u003c\/p\u003e\n\n\u003cp\u003eCleaning-in-place (CIP) capabilities\u003c\/p\u003e\n\n\u003cp\u003eThis agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.\u003c\/p\u003e\n\n\u003cp\u003eRegular cleaning can also increase column lifetime.\u003c\/p\u003e\n\n\u003cp\u003eChoose from 3 column sizes\u003c\/p\u003e\n\n\u003cp\u003eSuperdex™ 200 Increase columns are available pre-packed in three different sizes, to suit varying application needs (Table 1-1).\u003c\/p\u003e\n\n\u003ctable class=\"table table-striped\"\u003e\n\t\u003cthead\u003e\n\t\u003c\/thead\u003e\n\t\u003ctbody\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eColumn\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eRecommended sample volume\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eRecommended operating flow\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eEfficiency (N\/m)\u003c\/strong\u003e\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u003cstrong\u003eApplications \u003c\/strong\u003e\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eSuperdex™ 200 Increase 3.2\/300\u003c\/td\u003e\n\t\t\t\u003ctd\u003e4 to 50 µL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e0.075 mL\/min\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u0026gt;48,000\u003c\/td\u003e\n\t\t\t\u003ctd\u003eHigh-resolution analysis and purification of small sample volumes, especially when sample amount is limited and low buffer consumption is important.\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eSuperdex™ 200 Increase 10\/300 GL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e25 to 500 µL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e0.75 mL\/min\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u0026gt; 48,000\u003c\/td\u003e\n\t\t\t\u003ctd\u003eStandard for high-resolution preparative purification and analysis of small volumes of proteins, especially monoclonal antibodies\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003eSuperdex™ 200 Increase 5\/150 GL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e4 to 50 µL\u003c\/td\u003e\n\t\t\t\u003ctd\u003e0.45 mL\/min\u003c\/td\u003e\n\t\t\t\u003ctd\u003e\u0026gt; 42,000\u003c\/td\u003e\n\t\t\t\u003ctd\u003eRapid purity check, homogeneity analysis and screening of proteins, especially monoclonal antibodies.\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\n\n\u003cp\u003eIn addition to their use for antibody purification, Superdex™ 200 Increase pre-packed columns are also used in a variety of other applications, including:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31393998\/\" target=\"_blank\"\u003estructural analysis of Salmonella ATPase\u003c\/a\u003e ligand-bound states by SEC-MAL\u003c\/li\u003e\n\t\u003cli\u003eanalysis of \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27309808\/\" target=\"_blank\"\u003eprotein oligomerization states\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003estudy of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4330383\/\" target=\"_blank\"\u003ereplicative helicase machinery\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003eexamination of the structure, assembly, and function of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4315244\/\" target=\"_blank\"\u003ecytoplasmic pore filaments\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003einitiation of pulsed gene expression by \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4847170\/\" target=\"_blank\"\u003eLytTR transcription regulators\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cstrong\u003eReplacements for Superdex™ 200 10\/300 GL and 5\/150 GL columns\u003c\/strong\u003e\u003cbr\u003e\nSuperdex™ 200 increase columns replaced Cytiva’s popular Superdex 200 10\/300 GL (product code 17517501) and Superdex 200 5\/150 GL (product code 28906561).\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16542-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17491-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"3.2 × 300 mm","offer_id":45982034624591,"sku":"28990946","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 x 150 mm","offer_id":45982034657359,"sku":"28990945","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"10 x 300 mm","offer_id":45982034690127,"sku":"28990944","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-3789-original-png_36472d6d-babc-4711-a9f3-3856862a9375.png?v=1785957389","url":"https:\/\/www.danyel.co.il\/products\/superdex-200-increase-small-scale-sec-columns-28990944","provider":"danyel biotech","version":"1.0","type":"link"}