{"product_id":"sp-sepharose-fast-flow-cation-exchange-chromatography-resin-17072910","title":"SP Sepharose™ Fast Flow cation exchange chromatography resin","description":"\u003cp\u003eSP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWell established strong cation exchanger.\u003c\/li\u003e\n\u003cli\u003eBased on Sepharose Fast Flow ion exchange resin.\u003c\/li\u003e\n\u003cli\u003eHigh chemical stability enables proven CIP and sanitization protocols.\u003c\/li\u003e\n\u003cli\u003eHydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.\u003c\/li\u003e\n\u003cli\u003eFulfills industrial demands for security of supply, robust performance, and regulatory support.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStreamlined scale-up\u003c\/p\u003e\u003cp\u003eSP Sepharose Fast Flow uses the Sepharose Fast Flow ion exchange resin, which is well established in industrial downstream processing. The highly crosslinked agarose resin enables high flow rates and high productivity.\u003c\/p\u003e\u003cp\u003eComposed of crosslinked 6% agarose beads with sulphopropyl (SP) strong cation exchange groups, this resin has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.\u003c\/p\u003e\u003cp\u003eScale-up with these SP media (resins) is straightforward. Scale-up is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, PreDictor RoboColumns™, and HiTrap columns.\u003c\/p\u003e\u003cp\u003eFor even faster flow rates and better binding capacity in scale-up, Capto S and other Capto resins are a good option.\u003c\/p\u003e\u003cp\u003eSuccessful use – research scale\u003c\/p\u003e\u003cp\u003eSP Sepharose Fast Flow media (resins) are used in a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eTwo-step ion exchange chromatography of \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/17928929\/\"\u003ehuman calprotectin from neutrophils for structural studies\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurification of \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/9624183\/\"\u003eAPT1 from rat liver cytosol\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurification of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC106202\/\"\u003ea manganese peroxidase from \u003ci\u003ePanaeolus sphinctrinus\u003c\/i\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCompatible empty columns – research scale\u003c\/p\u003e\u003cp\u003eCytiva's solutions include a variety of empty columns for research scale. SP Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.\u003c\/p\u003e\u003cp\u003eCompatible empty columns – bioprocess scale\u003c\/p\u003e\u003cp\u003eFor process-scale applications, Cytiva recommends AxiChrom columns. SP Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.\u003c\/p\u003e\u003cp\u003ePrepacked columns – research scale\u003c\/p\u003e\u003cp\u003eThis strong cation exchange resin is available in the following prepacked formats:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eHiTrap SP FF columns – convenient for easy use with a syringe, peristaltic pump, or chromatography system such as ÄKTA system\u003c\/li\u003e\n\u003cli\u003eHiScreen SP FF columns for scale up and process development.\u003c\/li\u003e\n\u003cli\u003eHiPrep SP FF columns for convenient scale-up.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003ePrepacked columns – bioprocess scale\u003c\/p\u003e\u003cp\u003ePrepacked ReadyToProcess columns are available for bioprocess applications.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-12944-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/71500964.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"5 L","offer_id":45982042423375,"sku":"17072904","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"10 L","offer_id":45982042456143,"sku":"17072905","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"25 mL","offer_id":45982042488911,"sku":"17072910","price":536.0,"currency_code":"ILS","in_stock":true},{"title":"300 mL","offer_id":45982042521679,"sku":"17072901","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Standard","offer_id":45982042554447,"sku":"17072960","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7578-original-png_55106a6b-b824-4977-8ad3-2838c8dbec0c.png?v=1785957631","url":"https:\/\/www.danyel.co.il\/products\/sp-sepharose-fast-flow-cation-exchange-chromatography-resin-17072910","provider":"danyel biotech","version":"1.0","type":"link"}