{"product_id":"smarter-human-sctcr-a-b-profiling-kit-634432","title":"SMARTer® Human scTCR a\/b Profiling Kit","description":"\u003cp\u003eThe SMARTer Human TCR a\/b Profiling Kit enables users to analyze T-cell receptor (TCR) diversity from single T cells that have been sorted into a 96-well plate. As the name suggests, the kit can be used to generate data for both alpha- and beta-chain diversity. The kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of TCR transcripts. Included in the kit are primers that incorporate Illumina-specific adaptor sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFlexible workflow: Illumina-ready libraries from FACS or manually sorted single cells\u003c\/li\u003e\n\u003cli\u003eEase of use: optimized indexing allows for pooling 96 cells into twelve libraries which can be further multiplexed for running in a single flow-cell lane\u003c\/li\u003e\n\u003cli\u003eSensitivity: RACE-based approach allows for the detection of low-abundance TCR variants\u003c\/li\u003e\n\u003cli\u003eSpecificity: full-length reads, with the majority of reads on target and accurate pairing information\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHuman TCR repertoire analysis in single cells (TCR-alpha and TCR-beta subunits)\u003cbr\u003eTCR alpha-beta pairing determination in single cells\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST1391\/ST1391-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/SMARTer\/SMARTer%20Human%20scTCR%20ab%20Profiling%20Kit%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634431-634432.pdf\"\u003eSMARTer Human scTCR a\/b Profiling Kit Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"480 Rxns","offer_id":45986054897743,"sku":"634432","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/634432-SMARTer-Human-scTCR-a-b-Profiling-Kit-product-600.jpg?v=1782802808","url":"https:\/\/www.danyel.co.il\/products\/smarter-human-sctcr-a-b-profiling-kit-634432","provider":"danyel biotech","version":"1.0","type":"link"}