{"product_id":"smarter-human-bcr-igg-igm-h-k-l-profiling-kit-634467","title":"SMARTer® Human BCR IgG IgM H\/K\/L Profiling Kit","description":"\u003cp\u003eThe SMARTer Human BCR IgG IgM H\/K\/L Profiling Kit enables users to analyze B-cell receptor (BCR) repertoires from total RNA samples. This kit can generate libraries from 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) and 1 to 100 ng of total RNA from B cells. This kit may be used for other sample types, but the protocol may need to be modified. The kit can be used to generate data for both heavy and light chains (kappa and lambda) of human immunoglobulin IgG and IgM.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eInput: 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) or 1 to 100 ng of total RNA from B cells\u003c\/li\u003e\n\u003cli\u003eUMI-based correction for removal of reads derived from PCR error or duplication and\/or sequencing errors\u003c\/li\u003e\n\u003cli\u003eSensitive and specific clonotype detection with optimized cDNA library generation\u003c\/li\u003e\n\u003cli\u003eAccurate amplification of human IgG subclasses and identification via sequencing in a majority of cases\u003c\/li\u003e\n\u003cli\u003eOptimized PCR pooling strategy for highly sensitive detection of different chains from the same sample\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHuman BCR repertoire analysis (heavy and light [kappa and lambda] chains)\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST2130\/ST2130-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/SMARTer\/SMARTer%20Human%20BCR%20IgG%20IgM%20HKL%20Profiling%20Kit%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"48 Rxns","offer_id":45986055487567,"sku":"634467","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/634467-SMARTer-Human-BCR-IgG-IgM-HKL-Profiling-Kit-product-600.jpg?v=1782802832","url":"https:\/\/www.danyel.co.il\/products\/smarter-human-bcr-igg-igm-h-k-l-profiling-kit-634467","provider":"danyel biotech","version":"1.0","type":"link"}