{"product_id":"smart-seq-total-rna-single-cell-library-prep-with-zapr-depletion-634361","title":"SMART-Seq® Total RNA Single Cell Library Prep with ZapR® Depletion","description":"\u003cp\u003eSMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) generates strand-specific RNA-seq libraries for Illumina sequencing from 1–1,000 sorted cells or 10 pg–10 ng of purified total RNA. The kit was specifically designed to deliver highly sensitive and reproducible data from single cells while keeping the workflow short and user friendly. The kit does not require additional rRNA removal methods or kits and produces sequencing libraries that retain strand-of-origin information. Enough material is provided with this kit to perform up to 96 reactions.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSimple workflow to generate stranded Illumina sequencing-ready libraries in ~6.5 hours\u003c\/li\u003e\n\u003cli\u003eAppropriate for analysis of 1–1,000 intact mammalian cells or 10 pg–10 ng of low- or high-quality mammalian total RNA\u003c\/li\u003e\n\u003cli\u003eReproducible, accurate detection of full-length coding and noncoding transcripts from single cells or total RNA\u003c\/li\u003e\n\u003cli\u003eComparable sensitivity to our gold-standard SMART-Seq mRNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eGeneration of Illumina sequencing-ready libraries in 7 hours from small quantities of mammalian cells (1–1,000 cells) or total RNA (10 pg–10 ng)\u003cbr\u003eWhole transcriptome sequencing with strand-of-origin information\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST2670\/ST2670-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/SMART\/SMART-Seq%20Total%20RNA%20Single%20Cell%20Library%20Prep%20with%20ZapR%20Depletion%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634360-634361-634362.pdf\"\u003eSMART-Seq Total RNA Single Cell Library Prep with ZapR Depletion Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003ereagents for generating stranded sequencing libraries, including cDNA synthesis and amplification, ZapR-mediated ribosomal RNA depletion, and Illumina-compatible library preparation\u003c\/p\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634752\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (1–96)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634753\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (97–192)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634754\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (193–288)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634755\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (289–384)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634756\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (1–24)\u003c\/td\u003e\n\u003ctd\u003e24 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"96 Rxns","offer_id":45986054045775,"sku":"634361","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/SMART-Seq_Total_RNA-Single-Cell-fig-1-v3.jpg?v=1782802776","url":"https:\/\/www.danyel.co.il\/products\/smart-seq-total-rna-single-cell-library-prep-with-zapr-depletion-634361","provider":"danyel biotech","version":"1.0","type":"link"}