{"product_id":"smart-seq-human-bcr-with-umis-634776","title":"SMART-Seq® Human BCR (with UMIs)","description":"\u003cp\u003eSMART-Seq Human BCR (with UMIs) generates oligo(dT)-primed, full-length mRNA-seq libraries for all BCR heavy (IgG\/M\/D\/E\/A) and light (IgK\/L) chains. The incorporation of unique molecular identifiers (UMIs) provides greater accuracy for quantitative gene expression analysis across samples while controlling for PCR errors and amplification biases. Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index Kits (Cat. # 634752–56). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eComplete BCR repertoire profiling: includes all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains\u003c\/li\u003e\n\u003cli\u003eSupports various inputs: high-integrity RNA from PBMCs (10 ng–1 µg), B cells (1–100 ng), spleen or bone marrow (10 ng), and whole blood (100 ng)\u003c\/li\u003e\n\u003cli\u003eStreamlined workflow: primer pools for heavy and light immunoglobulin chains and enzyme premixes provided\u003c\/li\u003e\n\u003cli\u003eIncreased accuracy: UMI-based analysis controls for PCR duplicates and errors, correcting for artifacts in clonotype diversity and abundance assessments\u003c\/li\u003e\n\u003cli\u003eFlexible: sequence either the full-length V(D)J region or the CDR3 only and multiplex with up to 384 UDIs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST2500\/ST2500-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/SMART\/SMART-Seq%20Human%20BCR%20%28with%20UMIs%29%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634776.pdf\"\u003eSMART-Seq Human BCR (with UMIs) Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634752\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (1–96)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634753\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (97–192)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634754\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (193–288)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634755\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (289–384)\u003c\/td\u003e\n\u003ctd\u003e96 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e634756\u003c\/td\u003e\n\u003ctd\u003eUnique Dual Index Kit (1–24)\u003c\/td\u003e\n\u003ctd\u003e24 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"4 x 96 Rxns","offer_id":45986057355343,"sku":"634776","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Smart-seq_Human_BCR_w_UMI_9229_v3.jpg?v=1782802949","url":"https:\/\/www.danyel.co.il\/products\/smart-seq-human-bcr-with-umis-634776","provider":"danyel biotech","version":"1.0","type":"link"}