{"product_id":"seracare-seraseq-msi-reference-panel-mix-0710-1676","title":"Seraseq® MSI Reference Panel Mix","description":"\u003cp\u003eMicrosatellites are regions of DNA repeats with different lengths, i.e., instability, highlighting DNA mismatch repair gene deficiencies. Typical lengths for these DNA repeats are between 1-6 base pairs and vary person to person such that each person has a set length of these microsatellites in their genome. Measurements of MSI have traditionally been performed using qPCR\/CE analysis methods, but new methodologies are now available that includes digital droplet PCR (ddPCR) as well as Next Generation Sequencing (NGS). Microsatellite instability status has been linked to favorable outcomes in I-O treatment response by patients with diseases such as Lynch Syndrome and colorectal cancer. Hence MSI measurements have become an important biomarker in immuno-oncology therapeutics.\u003c\/p\u003e\u003cp\u003eLGC SeraCare has developed microsatellite instability (MSI) reference materials containing the key mono- and dinucleotide biomarkers typically analyzed in molecular MSI assays – NR-21, NR-24, BAT-25, BAT-26 and MONO-27. These markers are blended at two different allele frequency levels – AF5% and AF20% - to support MSI assay LoD determinations and accurate MSI detection. These products are offered as tumor-normal matched pairs, with variants precisely quantitated by digital PCR as well as qPCR\/CE against a highly characterized genomic DNA from a background WT cell line (GM24385) determined as microsatellite stable (MSS).\u003c\/p\u003e\u003cp\u003eFEATURES AND BENEFITS\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003ePlasmid-based spike-ins of 5 key MSI biomarkers typically analyzed in molecular assays that measure MSI in cancer patent samples\u003c\/li\u003e\n\u003cli\u003eOffered as tumor-normal matched pairs per AF level\u003c\/li\u003e\n\u003cli\u003eEach biomarker blended at two AF levels – 5% and 20% - to support assay LoD determinations and accurate MSI detection in patient samples\u003c\/li\u003e\n\u003cli\u003eVariant AFs quantitated by ddPCR and qPCR assays\u003c\/li\u003e\n\u003cli\u003eNormal background DNA is a highly characterized GM24385 human genomic DNA known to be microsatellite stable (MSS)\u003c\/li\u003e\n\u003cli\u003eManufactured within cGMP compliant and ISO 13485 certified facilities\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e# of Variants\u003c\/td\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e20 ng\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFill Volume\u003c\/td\u003e\n\u003ctd\u003e15 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal DNA\u003c\/td\u003e\n\u003ctd\u003e300 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e# of Vials\u003c\/td\u003e\n\u003ctd\u003e2 (tumor-normal matched)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMatrix\u003c\/td\u003e\n\u003ctd\u003eTE Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMAP4K3 (intron13)\u003c\/td\u003e\n\u003ctd\u003e39536690\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/ps-mkt-00534_0710-1670.0710-2236.0710-1675.0710-1676_seraseq_msi-reference_materials.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq MSI Reference Materials Product Sheet (Product Sheet)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/ds-mkt-00555-0710-16700710-16750710-1676-seraseq_msi_reference_materials_fnl-4.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq MSI Reference Materials (Data Sheet)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/pi-0710-1675.1676-seraseq-msi-ref-panel-mix-af5-af20.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq MSI Reference Panel Mix AF5% and AF20% (Package Insert)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/nrl-0710-1675-1676-seraseq-msi-reference-panel.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq MSI Reference Panel Non-Requirement Letter (SDS Non-Requirement Letter)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. no.\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003cth\u003eAllele Frequency\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e0710-1676\u003c\/td\u003e\n\u003ctd\u003eSeraseq® MSI Reference Panel Mix AF20%\u003c\/td\u003e\n\u003ctd\u003e2 x 15 µL\u003c\/td\u003e\n\u003ctd\u003e20%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0710-1675\u003c\/td\u003e\n\u003ctd\u003eSeraseq® MSI Reference Panel Mix AF5%\u003c\/td\u003e\n\u003ctd\u003e2 x 15 µL\u003c\/td\u003e\n\u003ctd\u003e5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"SeraCare","offers":[{"title":"AF20%","offer_id":46798511341647,"sku":"0710-1676","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"AF5%","offer_id":46798511374415,"sku":"0710-1675","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/0710-1676.jpg?v=1789655843","url":"https:\/\/www.danyel.co.il\/products\/seracare-seraseq-msi-reference-panel-mix-0710-1676","provider":"danyel biotech","version":"1.0","type":"link"}