{"product_id":"seracare-seraseq-gdna-tmb-mix-0710-1326","title":"Seraseq® gDNA TMB Mix","description":"\u003cp\u003eIn immuno-oncology, the goal is to enable a patient’s immune system to locate and eliminate cancerous cells. The immune system may normally protect against cancer – except when cancers manage to evade it – since immunocompromised individuals have a higher likelihood of developing cancer. Checkpoint inhibitors, which have recently improved the prognosis for a significant number of cancer patients, work by blocking inhibitory molecules such as PD-L1 and CTLA-4 that may be allowing cancers to evade the adaptive immune system. However, by taking the brakes off the immune system, the use of checkpoint inhibitors is associated with a significant risk of developing autoimmune disease. Additionally, frequently checkpoint inhibitors are not effective.\u003c\/p\u003e\u003cp\u003eIn order to maximize the safety and efficacy of checkpoint inhibitors, it is beneficial to identify patients who are likely to respond to their use. Since the adaptive immune system can detect changes to proteins, it is thought that the number of somatic mutations that lead to such changes may be correlated with efficacy. The metric of non-silent somatic mutations per megabase of coding DNA has been termed Tumor Mutational Burden (TMB), which has shown some correlation to the efficacy of checkpoint inhibitors. Assessments of TMB are being added to many NGS assays to enable their use for patient selection in I-O clinical trials, and perhaps as companion diagnostics to these therapies. However, it is also known that TMB scores can differ significantly between assays and especially around levels that may be clinical decision points.\u003c\/p\u003e\u003cp\u003eTo improve and harmonize the measurement of TMB, we have generated and characterized tumor-normal human cell lines with different TMB scores by whole exome sequencing for use in validating targeted NGS panel-based TMB assays, and as controls for TMB measurements in I-O clinical trial projects.\u003c\/p\u003e\u003cp\u003eThe TMB reference materials are formated as genomic DNA (purified mix) and\/or FFPE (requires extraction).\u003c\/p\u003e\u003cp\u003egDNA TMB Reference Standards:\u003cbr\u003eReference standards are made from human lung cancer cell lines and their matched “normal” cell lines from peripheral blood of the same patients.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e100% tumor-normal matched reference standards\u003c\/li\u003e\n\u003cli\u003epurified gDNA mix, ready as input into WES or targeted NGS library preparation\u003c\/li\u003e\n\u003cli\u003erange of TMB scores\u003c\/li\u003e\n\u003cli\u003emanufactured in GMP-compliant and ISO 13485 certified facilities\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFill Volume\u003c\/td\u003e\n\u003ctd\u003e10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e50 ng\/µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/PS-MKT-00504-04-0710-1326.0710-1325.0710-1586.0710-1324.0710-1323.0710-2087.0710-2088.0710-2089.0710-2090.0710-1310.0710-1308.0710-1618.0710-1309.0710-1307.Seraseq_gDNA_ctDNA_and_FFPE_TMB_Products.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq gDNA, ctDNA and FFPE TMB Product Sheet (Product Sheet)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/ds-0710-13230710-13240710-13250710-1326-seraseq-gdna-tmb-mix-score-7-9-20-26.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq gDNA TMB Mix (Data Sheet)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/pi-0710-1323.1324.1325.1326-seraseq-gdna-ffpe-tmb-mix.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq gDNA TMB Mix Products (Package Insert)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/nrl-0710-2463.1326.1325-seraseq-gdna-tmb.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq gDNA TMB Non-Requirement Letter (SDS Non-Requirement Letter)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.seracare.com\/globalassets\/seracare-resources\/nrl-0710-1586.1324.1323seraseq-gdna-mix.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSeraseq gDNA TMB Mix Score Non-Requirement Letter (SDS Non-Requirement Letter)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. no.\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003cth\u003eTMB Score\u003c\/th\u003e\n\u003cth\u003eTotal Mass\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e0710-1326\u003c\/td\u003e\n\u003ctd\u003eSeraseq® gDNA TMB Mix Score 7\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003ctd\u003e7.2 ± 0.2*\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0710-1325\u003c\/td\u003e\n\u003ctd\u003eSeraseq® gDNA TMB Mix Score 9\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003ctd\u003e9.5 ± 0.4\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0710-1586\u003c\/td\u003e\n\u003ctd\u003eSeraseq® gDNA TMB Mix Score 13\u003c\/td\u003e\n\u003ctd\u003e2 x 10 µL\u003c\/td\u003e\n\u003ctd\u003e12.6 ± 0.02*\u003c\/td\u003e\n\u003ctd\u003e2x 500 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0710-1324\u003c\/td\u003e\n\u003ctd\u003eSeraseq® gDNA TMB Mix Score 20\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003ctd\u003e20.1 ± 0.2*\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0710-1323\u003c\/td\u003e\n\u003ctd\u003eSeraseq® gDNA TMB Mix Score 26\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003ctd\u003e25.8 ± 0.5*\u003c\/td\u003e\n\u003ctd\u003e2 x 500 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"SeraCare","offers":[{"title":"Score 7","offer_id":46798510948431,"sku":"0710-1326","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Score 9","offer_id":46798510981199,"sku":"0710-1325","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Score 13","offer_id":46798511013967,"sku":"0710-1586","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Score 20","offer_id":46798511046735,"sku":"0710-1324","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Score 26","offer_id":46798511079503,"sku":"0710-1323","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/0710-1326.jpg?v=1789655832","url":"https:\/\/www.danyel.co.il\/products\/seracare-seraseq-gdna-tmb-mix-0710-1326","provider":"danyel biotech","version":"1.0","type":"link"}