{"product_id":"sera-mag-carboxylate-modified-magnetic-beads-speedbeads-45152105050250","title":"Sera-Mag™ Carboxylate-Modified Magnetic Beads \u0026 SpeedBeads","description":"\u003cp\u003eWhat are the Sera-Mag™ Carboxylate-Modified Magnetic Beads \u0026amp; SpeedBeads? Sera-Mag™ Carboxylate-Modified Magnetic Beads \u0026amp; SpeedBeads have free carboxyl groups on the surface for convenient covalent coupling of target molecules (proteins, peptides, and amine-modified ligands) via primary amines (NH 2 ).\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWide application range: Carboxylic groups on the surface form covalent amide bonds with proteins, peptides or amine-modified ligands for use in many downstream applications including protein purification, DNA sample preparation and clean-up, protenomicsand immunoassays.\u003c\/li\u003e\n\u003cli\u003eConvenient one-step or two-step coupling: Carbodiimide chemistry allows rapid formation of stable bonds to minimize ligand leaching.\u003c\/li\u003e\n\u003cli\u003eFast magnetic separation: Available in original Sera-Mag™ format or Sera-Mag™ SpeedBeads version for faster magnetic response and shorter assay times in clinical diagnostic tests.\u003c\/li\u003e\n\u003cli\u003eHigh performance: Excellent sensitivity and low nonspecific binding for greater accuracy.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eSera-Mag™ SpeedBead carboxylate-modified magnetic particles now available in 1 µm and 3 µm nominal diameters.\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003ca class=\"ge-button -fill--transparent -size--large -color--blue -padding--noleft -margin--noleft\" href=\"\/solutions\/genomics\/sequencing\/sera-mag\"\u003eRequest a free sample\u003c\/a\u003e\u003c\/p\u003e\n\n\u003cp\u003eFind out which magnetic bead chemistry meets your application requirements in \u003ca href=\"\/news-center\/magnetic-beads-a-simple-guide-10001\"\u003eMagbeads 101: A guide to choosing and using magnetic beads\u003c\/a\u003e and explore ways to optimize your magnetic bead workflow in \u003ca href=\"\/solutions\/genomics\/knowledge-center\/magnetic-bead-guide\/magnetic-bead-basics\"\u003eScientists guide to magnetic beads\u003c\/a\u003e\u003c\/p\u003e\n\n\u003cp\u003eWhat is the difference between E3 and E7 bead?\u003c\/p\u003e\n\n\u003cp\u003eE3 and E7 refer to the different manufacturing process for the beads. E3 and E7 beads behave similarly and we continue to provide both bead types to our customers for test and validation in their chosen application.\u003c\/p\u003e\n\n\u003cp\u003eHow to covalently couple proteins to carboxylate-modified magnetic beads\u003c\/p\u003e\n\n\u003cp\u003eThe convenient “one-step” protocol for covalent coupling of proteins to Sera-Mag™ carboxylate-modified particles or Sera-Mag™ SpeedBeads carboxylate-modified particles involves the following steps\u003c\/p\u003e\n\n\u003col class=\"-font--body\"\u003e\n\t\u003cli\u003ePrepare a reagent mixture containing MES buffer, protein solution, and magnetic particles, and mix for 15 minutes at room temperature on a mixing wheel or other device.\u003c\/li\u003e\n\t\u003cli\u003eAdd freshly prepared EDAC solution at the pre-determined optimal concentration, and mix for one hour at room temperature on a mixing wheel or other device.\u003c\/li\u003e\n\t\u003cli\u003ePellet particles by centrifugation (10 to 30 minutes in a standard microcentrifuge) and decant the supernatant.\u003c\/li\u003e\n\t\u003cli\u003ePerform two washes with MES buffer or a high pH buffer of your choice, pelleting particles by centrifugation and using ultrasonication to resuspend pellets between washes.\u003c\/li\u003e\n\t\u003cli\u003eResuspend final pellet to desired % solids with a buffer such as MES that does not contain blocking proteins.\u003c\/li\u003e\n\t\u003cli\u003ePerform a BCA protein assay to determine the amount of protein bound on the particles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eHave you considered your magnetic separation rack?\u003c\/p\u003e\n\n\u003cp\u003eCytiva’s Magnetic Separation Rack 15 mL is a 3D printed, magnetized tube rack with a strong, removable magnetic force and easy pipette access. It facilitates high capture, retention, and release of magnetic beads from various liquid sample media, allowing high-throughput, parallel processing of up to six 15 mL samples.\u003c\/p\u003e\n\n\u003cp\u003e\u003ca class=\"ge-button -fill--transparent -size--large -color--blue -padding--noleft -margin--noleft\" href=\"\/shop\/protein-analysis\/protein-sample-preparation\/protein-enrichment\/magnetic-separation-racks-p-05937\"\u003eFind out more about magnetic separation racks \u003c\/a\u003e\u003c\/p\u003e\n\n\u003cp\u003eCheck out \u003ca href=\"\/solutions\/diagnostics\/diagnostic-services\"\u003eCytiva Diagnostic Services\u003c\/a\u003e, helping you build and accelerate the development of smarter molecular and immunodiagnostic assays.\u003c\/p\u003e\n\n\u003cp\u003eOur ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17286-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29513474.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 µm \/ 15 mL (45152105050250)","offer_id":45982216519759,"sku":"45152105050250","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 15 mL (65152105050250)","offer_id":45982216552527,"sku":"65152105050250","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 15 mL (24152105050250)","offer_id":45982216585295,"sku":"24152105050250","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 15 mL (44152105050250)","offer_id":45982216618063,"sku":"44152105050250","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 100 mL (45152105050350)","offer_id":45982216650831,"sku":"45152105050350","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 100 mL (24152105050350)","offer_id":45982216683599,"sku":"24152105050350","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 100 mL (44152105050350)","offer_id":45982216716367,"sku":"44152105050350","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 100 mL (65152105050350)","offer_id":45982216749135,"sku":"65152105050350","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 1000 mL (44152105050450)","offer_id":45982216781903,"sku":"44152105050450","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 1000 mL (24152105050450)","offer_id":45982216814671,"sku":"24152105050450","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 1000 mL (45152105050450)","offer_id":45982216847439,"sku":"45152105050450","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 µm \/ 1000 mL (65152105050450)","offer_id":45982216880207,"sku":"65152105050450","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"3 µm \/ 1 mL","offer_id":45982216912975,"sku":"29729998","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"3 µm \/ 10 mL","offer_id":45982216945743,"sku":"29729997","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"3 µm \/ 100 mL","offer_id":45982216978511,"sku":"29730063","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-47940-original-png_fd7fdb5a-6572-4ad9-bedd-542d28aadc03.png?v=1785959118","url":"https:\/\/www.danyel.co.il\/products\/sera-mag-carboxylate-modified-magnetic-beads-speedbeads-45152105050250","provider":"danyel biotech","version":"1.0","type":"link"}