{"product_id":"sephadex-g-50-dna-grade-f-17057301","title":"Sephadex G-50 DNA Grade F","description":"\u003cp\u003eSephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor purification of DNA from small molecules by size exclusion\u003c\/li\u003e\n\u003cli\u003eTested to ensure reproducibly high recovery of DNA\u003c\/li\u003e\n\u003cli\u003eSephadex G-50 DNA Grade is used in various G-50 columns, such as AutoSeq G-50 columns, ProbeQuant G-50 Micro Columns, MicroSpin G-50 Columns, and NICK Columns\u003c\/li\u003e\n\u003cli\u003eSuitable for researchers who prefer to prepare their own columns for purifying nucleic acids\u003c\/li\u003e\n\u003cli\u003eExcellent for rapid purification of newly synthesized oligonucleotides \u0026gt; 10-mers, including oligonucleotide probes, in 100 to 150 µL of deprotection solution using spin-column chromatography\u003c\/li\u003e\n\u003cli\u003eUseful for desalting or buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-50-dna-grade-p-00113\"\u003eSephadex G-50\u003c\/a\u003e is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eAlternatively, \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-25-dna-grade-p-00054\"\u003eSephadex G-25\u003c\/a\u003e can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eBoth types of Sephadex are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction.\u003c\/p\u003e\n\n\u003cp\u003eMicroSpin columns packed with \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-50-dna-grade-p-00113\"\u003eSephadex G-50 DNA Grade\u003c\/a\u003e are suitable for the rapid purification of DNA, for use in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003c\/p\u003e\n\n\u003cp\u003eSephadex G-25 DNA Grade and Sephadex G-50 DNA Grade are sold separately.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"25 g","offer_id":45982267605071,"sku":"17057301","price":784.0,"currency_code":"ILS","in_stock":true},{"title":"100 g","offer_id":45982267637839,"sku":"17057302","price":2486.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-5476-original-png_f7d8e029-3ae3-4bea-8030-7e7b46241a84.png?v=1785959635","url":"https:\/\/www.danyel.co.il\/products\/sephadex-g-50-dna-grade-f-17057301","provider":"danyel biotech","version":"1.0","type":"link"}