{"product_id":"sbeadex-pathogen-nucleic-acid-purification-kit-no-protease-no-dangerous-goods-sbead-004","title":"sbeadex Pathogen Nucleic Acid Purification Kit - No Protease; No Dangerous Goods","description":"\u003cp\u003eThe sbeadex Pathogen Nucleic Acid Purification Kit without protease and dangerous goods is a cutting-edge solution for fast and reliable isolation of highly purified DNA and RNA from various pathogenic bacteria, viruses and yeasts.\u003c\/p\u003e\u003cp\u003eThe sbeadex Technology\u003c\/p\u003e\u003cp\u003eThis kit leverages the power of sbeadex magnetic bead technology. These superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel two-bind \/ two-wash mechanism that ensures effective removal of impurities from the sample matrix. The second wash buffer in the kit is ethanol-free, eliminating any traces of ethanol that could potentially inhibit downstream reactions. Additionally, the sbeadex technology eliminates the need for a drying step before elution, further enhancing DNA quality.\u003c\/p\u003e\u003cp\u003eThe inclusion of Proteinase K facilitates efficient cell disruption and nucleic acid release with certain sample material and safeguards its integrity. The Proteinase K enzyme enhances the efficiency of nucleic acid extraction by digestion of proteins in the cell lysate. This addition helps produce higher DNA\/RNA yields and improves the stability of extracted nucleic acids.\u003c\/p\u003e\u003cp\u003esbeadex Pathogen Nucleic Acid Purification Kit\tCompetitor A\u003cbr\u003e \tN\tAverage Cq\tStDev\tAverage Cq\tStDev\u003cbr\u003eClean up sample\t12\t30.35\t0.14\t33.80\t0.15\u003cbr\u003eDirect spike-in sample\t3\t31.00\t0.44\t33.80\t0.12\u003c\/p\u003e\u003cp\u003eFigure 1:  Demonstration of no loss of RNA during the sbeadex Pathogen Nucleic Acid Purification Kit protocol.  500 copies of a 2019-COVID artificial RNA reference material were added to a sample (nasopharyngeal swab) and the sample processed with the sbeadex Pathogen Nucleic Acid Purification Kit. SARS-CoV-2 was detected in this preparation and a directly spiked positive control sample via a RT-qPCR at two separate Biosearch Technologies sites (reaction components described below). The concordant Cq values from both samples demonstrate no loss of sample due to the extraction step.\u003c\/p\u003e\u003cp\u003eThis is a ready-to-use kit designed to streamline the DNA and RNA purification process, making it an ideal choice for downstream applications such as:\u003c\/p\u003e\u003cp\u003ePCR \/ qPCR\u003c\/p\u003e\u003cp\u003eReal Time (RT) PCR \/ qRT-PCR\u003c\/p\u003e\u003cp\u003eNext Generation Sequencing (NGS)\u003c\/p\u003e\u003cp\u003eThe kit is compatible with a wide range of automation platforms, such as Thermo's KingFisher and Hamilton's Microlab Star or our oKtopure, and can be easily automated on various liquid handlers and purification platforms. It offers high flexibility in reaction volumes, making it suitable for high-throughput purifications.\u003c\/p\u003e\u003cp\u003eThis kit contains no dangerous goods ensuring an extra level of safety and regulatory compliance.\u003c\/p\u003e\u003cp\u003eThe sbeadex Pathogen testing kits have been used successfully with:\u003c\/p\u003e\u003cp\u003ePathogen\tExternal Control (Artificial Matrix)\u003cbr\u003eViral Targets\t \u003cbr\u003eSARS-CoV-2\tVTM1\u003cbr\u003eInfluenza A H3N2\tSwab2\u003cbr\u003eInfluenza B\tSwab2\u003cbr\u003eNovel Influenza A H1N1\tSwab2\u003cbr\u003eRespiratory Syncytial Virus (subtype A)\tSwab2\u003cbr\u003eRespiratory Syncytial Virus (subtype B)\tSwab2\u003cbr\u003eAdenovirus 4\tSwab2\u003cbr\u003eZika Virus\tPlasma3\u003cbr\u003eCytomegalovirus\tPlasma4\u003cbr\u003eHuman Papillomavirus-16\tMethanol-preserved cultured human cells containing full-length HPV episomal DNA5\u003cbr\u003eBacterial Targets\t \u003cbr\u003eBordetella pertussis\tSwab6\u003cbr\u003eChlamydophila pneumoniae\tSwab6\u003cbr\u003eChlamydophila psittaci\tSwab6\u003cbr\u003eCoxiella burnetii\tSwab6\u003cbr\u003eLegionella pneumophila\tSwab6\u003cbr\u003eMycoplasma pneumoniae\tSwab6\u003cbr\u003eMycobacterium tuberculosis\tSputum7\u003cbr\u003eCampylobacter jejuni\tStool8\u003cbr\u003eSalmonella enteritidis\tStool8\u003cbr\u003eYersinia enterocolitica\tStool8\u003cbr\u003eClostridium difficile\tStool8\u003cbr\u003eChlamydia trachomatis\tUrine9\u003cbr\u003eNeisseria gonorrhoeae\tUrine9\u003cbr\u003eStaphylococcus aureus\tExternal Run Control10\u003cbr\u003eOther Targets\u003cbr\u003eCandida albicans\tPositive Extraction Control11\u003cbr\u003eCompatible Matrices Tested\tSwabs (UTM®\/VTM)\u003cbr\u003eSputum\u003cbr\u003eSaliva\u003cbr\u003eBlood\u003cbr\u003ePlasma\u003cbr\u003eSerum\u003cbr\u003eUrine\u003cbr\u003eStool\u003cbr\u003eCerebrospinal Fluid\u003c\/p\u003e\u003cp\u003eTable 1: Pathogens that have already been tested using the sbeadex Pathogen Nucleic Acid Purification Kit.\u003c\/p\u003e\u003cp\u003eStandards used for testing\u003cbr\u003eAccuPlex™ SARS-CoV-2 Verification Panel - Full Genome, 0505-0168 (LGC SeraCare)\u003cbr\u003eAMPLIRUN® TOTAL RESPIRATORY VIRAL PANEL CONTROL (SWAB), MBTC020 (Vircell S.L.)\u003cbr\u003eAMPLIRUN TOTAL ZIKV\/DENV\/CHIKV CONTROL (PLASMA), MBTC023 (Vircell S.L.)\u003cbr\u003eAMPLIRUN TOTAL CMV CONTROL (PLASMA), MBTC018-R (Vircell S.L.)\u003cbr\u003eAccuTrak™ HPV Genotype Qualification Panel, QSH701 (2400-0161), (LGC SeraCare)\u003cbr\u003eAMPLIRUN TOTAL ATYPICAL BACTERIAL PNEUMONIA CONTROL (SWAB) MBTC022-R (Vircell S.L.)\u003cbr\u003eAMPLIRUN TOTAL MTB CONTROL (SPUTUM) MBTC013 (Vircell S.L.)\u003cbr\u003eAMPLIRUN TOTAL GASTROINTESTINAL BACTERIAL PANEL CONTROL (STOOL), MBTC021 (Vircell S.L.)\u003cbr\u003eAMPLIRUN TOTAL CT\/NG CONTROL (URINE), MBTC003 (Vircell S.L.)\u003cbr\u003eNATtrol™ S. aureus (MRSA) External Run Control, NATSAU(MRSA)-ERCM (Zeptometrix Corporation)\u003cbr\u003eNATtrol Candida\/TV Positive Control, NATCTVPOS-BD (Zeptometrix Corporation)\u003c\/p\u003e\u003cp\u003eNote: Other sample matrices may also be used with this kit. Further testing on other pathogens and matrices is in progress and this table will be updated accordingly.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRapid nucleic acid purification from various pathogens\u003c\/li\u003e\n\u003cli\u003eContains no dangerous goods, avoiding shipping problems to distinct destinations.\u003c\/li\u003e\n\u003cli\u003eHigh-quality sbeadex magnetic bead-based technology\u003c\/li\u003e\n\u003cli\u003eReady-to-use kit compatible with most robotic platforms\u003c\/li\u003e\n\u003cli\u003eSuitable for many downstream applications such as: PCR\/qPCR, RT-PCR\/qRT-PCR, and NGS\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe sbeadex Pathogen Nucleic Acid Purification Kit without protease and dangerous goods includes a Lysis Buffer, Binding Buffer, a variety of Wash Buffers, an Elution Buffer, sbeadex particle suspension solution containing EDTA.\u003c\/p\u003e\u003cp\u003eThis kit does not contain dangerous goods.\u003c\/p\u003e\u003cp\u003eValidated for research use only using AccuPlex™ reference material and is compatible with our COVID-19 detection kit.\u003c\/p\u003e\u003cp\u003eChoose between 96 or 960 preparations, or enquire for bulk options.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003eautomation sbeadex Pathogen Hamilton\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Binding-Buffer-SB\"\u003eBinding Buffer SB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Binding-buffer-SB-concentrate-for-Asia-Pacific\"\u003eBinding buffer SB concentrate for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Elutions-Buffer-AMP\"\u003eElutions Buffer AMP\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Elutions-Buffer-AMP-for-Asia-Pacific\"\u003eElutions Buffer AMP for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Lysis-Buffer-SB\"\u003eLysis Buffer SB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Lysis-Buffer-SB-for-Asia-Pacific\"\u003eLysis Buffer SB for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_PK-Solution-Protease-Solution\"\u003ePK Solution Protease Solution\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_PK-Solution-Protease-Solution-for-Asia-Pacific\"\u003ePK Solution Protease Solution for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Wash-Buffer-BN1\"\u003eWash Buffer BN1\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Wash-Buffer-BN1-concentrate-for-Asia-Pacific\"\u003eWash Buffer BN1 concentrate for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Wash-Buffer-PN1-FN1-TN1\"\u003eWash Buffer PN1 FN1 TN1\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Wash-Buffer-PN1-FN1-TN1-concentrate-for-Asia-Pacific\"\u003eWash Buffer PN1 FN1 TN1 concentrate for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Wash-Buffer-PN2-FN2-TN2-BLN-PLN\"\u003eWash Buffer PN2 FN2 TN2 BLN PLN\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_Wash-Buffer-PN2-FN2-TN2-BLN-PLN-for-Asia-Pacific\"\u003eWash Buffer PN2 FN2 TN2 BLN PLN for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_sbeadex-particle-suspension-plus-EDTA\"\u003esbeadex particle suspension plus EDTA\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBEAD-004_sbeadex-particle-suspension-plus-EDTA-for-Asia-Pacific\"\u003esbeadex particle suspension plus EDTA for Asia Pacific\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/protocol_SBEAD-004_automation-sbeadex-nucleic-acid-purification-kit-oktopure\"\u003eautomation sbeadex nucleic acid purification kit oktopure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/protocol_SBEAD-004_sbeadex-pathogen-nucleic-acid-purification-kit\"\u003esbeadex pathogen nucleic acid purification kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/protocol_SBEAD-004_sbeadex-pathogen-nucleic-acid-purification-kit-kingfisher-flex\"\u003esbeadex pathogen nucleic acid purification kit kingfisher flex\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_SBEAD-004_sbeadex-pathogen-nucleic-acid-purification-kit\"\u003esbeadex pathogen nucleic acid purification kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/data-sheet_SBEAD-004_sbeadex-pathogen-nucleic-acid-purification-kit\"\u003esbeadex pathogen nucleic acid purification kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_SBEAD-004_automation-sbeadex-Pathogen-Hamilton\"\u003eautomation sbeadex Pathogen Hamilton\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"NAP40-027-01","offer_id":45986466332751,"sku":"NAP40-027-01","price":2271.0,"currency_code":"ILS","in_stock":true},{"title":"NAP40-027-02","offer_id":45986466365519,"sku":"NAP40-027-02","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"NAP40-027-03","offer_id":45986466398287,"sku":"NAP40-027-03","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"NAP40-027-04","offer_id":45986466431055,"sku":"NAP40-027-04","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/sbeadex-Pathogen-Nucleic-Acid-Purification-Kit---No-Dangerous-Goods_a6ec5105-d0ac-4da5-82c2-955c22259425.jpg?v=1785956574","url":"https:\/\/www.danyel.co.il\/products\/sbeadex-pathogen-nucleic-acid-purification-kit-no-protease-no-dangerous-goods-sbead-004","provider":"danyel biotech","version":"1.0","type":"link"}