{"product_id":"sbeadex-lightning-nucleic-acid-purification-kit-modular-sbxlck","title":"sbeadex Lightning Nucleic Acid Purification Kit Modular","description":"\u003cp\u003eExplore the versatility of sbeadex Lightning chemistry\u003c\/p\u003e\u003cp\u003eDiscover the potential with our two core kit options, Core kit A and Core kit B. Each kit contains everything you need to follow our lightning protocol designed for efficient 3-step purification of high-quality DNA from plant and animal tissue lysates in as little as typically 5 minutes. You can complete the Core Kits by ordering individual components such as recommended lysis buffers, Protease K or Debris capture beads. Before ordering a Core Kit, we recommend using our Starter Kit to evaluate and determine the optimal composition of Core kit and individual components.\u003c\/p\u003e\u003cp\u003eCore Kit options\u003c\/p\u003e\u003cp\u003eCore Kit A: Recommended for most sample varieties and particularly effective for plant samples.\u003c\/p\u003e\u003cp\u003eCore Kit B: Optimized for specific sample types, especially mammalian tissue samples.\u003c\/p\u003e\u003cp\u003eWe highly recommend starting with our comprehensive Starter Kit to evaluate optimal lysis conditions and performance before progressing to one of our core kits.\u003c\/p\u003e\u003cp\u003eBoth kits embrace an environmentally friendly approach that reduces plastics, packaging, shipping costs, and hazardous waste.\u003c\/p\u003e\u003cp\u003eTo complete your sbeadex Lightning kit we offer a range of individual components that allow a flexible workflow optimization tailored to your specific sample types and needs.\u003c\/p\u003e\u003cp\u003eRevolutionary lightning technology\u003c\/p\u003e\u003cp\u003eOur patent pending sbeadex Lightning product line brings a new era of nucleic acid isolation with a superfast and straightforward 3-step protocol. Purify nucleic acids in just typically 5 minutes from a variety of plant and animal sample materials. This innovative technology cuts time by requiring only a binding buffer, our proprietary sbeadex particles, and an elution buffer. Despite that simplified and shortened protocol, sbeadex Lightning delivers highly pure DNA in high yields that easily draws level with popular purification kits.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eDNA purification: Ideal for downstream applications such as PCR\/qPCR, restriction analysis\/cloning or sequencing\/NGS.\u003c\/p\u003e\u003cp\u003eWide sample compatibility: Applicable to a diverse range of sample types, including plant and animal tissues (see product details).\u003c\/p\u003e\u003cp\u003eThe sbeadex Lightning technology\u003c\/p\u003e\u003cp\u003eThis kit leverages the power of sbeadex magnetic bead technology. These superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel one-step binding mechanism that ensures effective removal of impurities from the sample matrix. The recommended but optional wash step is performed with water, preventing organic\/alcoholic traces in the eluate that might inhibit enzymatic downstream applications. This eliminates the need for a drying step before elution, further enhancing DNA quality.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSave time and increase sample throughput: Superfast and convenient protocols that adapt seamlessly to automated high-throughput workflows\u003c\/li\u003e\n\u003cli\u003eFlexibility: Optimise nucleic acid purification from different sample types using our self-assembly kit solutions (2 core kits, 6 different lysis buffers and other optional components). Unsure which to choose? Start with our sbeadex Lightning Nucleic Acid Purification Starter Kit\u003c\/li\u003e\n\u003cli\u003eCost-savings: Benefit from a 3-step\/5-minute protocol that reduces overall lab costs (e.g. plastic consumables) and ensures reliable and reproducible results\u003c\/li\u003e\n\u003cli\u003eHigh DNA quality and yield: Attain highly purified DNA in high yields suitable for a wide range of downstream applications\u003c\/li\u003e\n\u003cli\u003eConvenience and stability: Ready-to-use reagents with high stability at room temperature\u003c\/li\u003e\n\u003cli\u003eSustainability and eco-friendliness: New, flexible kit format and shortened protocol reduce components to what is actually needed - reducing plastic, liquid and packaging waste\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eAutomation of sbeadex Lightning\u003c\/p\u003e\u003cp\u003eoKtopure\u003c\/p\u003e\u003cp\u003eThe sbeadex Lightning DNA purification protocol can be automated using our fully automated nucleic acid isolation platform, the oKtopure™ platform.\u003c\/p\u003e\u003cp\u003eFor experienced oKtopure users, the following two links to zip files contain a run file and three advanced mixing routines, one for each step of the protocol (bind, wash, elute). These are provided for both 100 µL and 200 µL of lysate input volume.\u003c\/p\u003e\u003cp\u003eThe table below provides summary information about the available oKtopure run files, including the total run time of each.\u003c\/p\u003e\u003cp\u003e100 µL input volume\t200 µL input volume\u003cbr\u003eRun file\tsbxlightning_100V_Elu50\tsbxlightning_200V_Elu100\u003cbr\u003eRun time (8 plates)\t1 hour 36 minutes\t1 hour 55 minutes\u003c\/p\u003e\u003cp\u003eWhen establishing sbeadex Lightning on your oKtopure, we recommend liaising with our automation experts to ensure that all instrument settings are appropriate for your sample types. Biosearch Technologies offer pilot projects to determine feasibility for challenging sample types and to address parameters such as customised sample input amounts and elution volumes.\u003c\/p\u003e\u003cp\u003eKingFisher Flex\u003c\/p\u003e\u003cp\u003eAutomation can also be achieved using the KingFisher Flex platform; we provide standard protocols that you can access below.\u003c\/p\u003e\u003cp\u003eThis zip file contains 18 different BindIt files (.bdz) and 18 corresponding report files (.pdf) that contain general information about the run file, the reagent information and details regarding the steps of the automated protocol.\u003c\/p\u003e\u003cp\u003eTo determine the most appropriate BindIt file (.bdz) for your application, you need to choose:\u003c\/p\u003e\u003cp\u003ethe relevant input volume – either 50 µL, 100 µL or 200 µL of lysate per well.\u003c\/p\u003e\u003cp\u003ethe desired elution time – 90 seconds, 300 seconds, or 600 seconds. Different plant species and sample types react differently to the elution time. In general, longer elution times guarantee that all available DNA is eluted, but for some sample types (particularly plants) the shorter elution times have shown to be sufficient.\u003c\/p\u003e\u003cp\u003ethe number of plates to be processed simultaneously – either one plate or two plates, depending on your laboratory setup.\u003c\/p\u003e\u003cp\u003eThe table below provides summary information about the available BindIt files, including the total run time of each.\u003c\/p\u003e\u003cp\u003eHamilton STAR\u003c\/p\u003e\u003cp\u003eAutomation can also be achieved using the Hamilton STAR™ platform. In our sbeadex Lightning DNA purification manual for Hamilton STAR we provide an overview of how to use the Hamilton STAR Lightning protocol along with example protocols for purification of nucleic acids from common plant and livestock tissue types. The protocol can be user adjusted using the protocol optimisation panel to meet requirements for specific sample types and methods.\u003c\/p\u003e\u003cp\u003eAlternative liquid handlers can also be used to automate the sbeadex Lightning chemistry; please contact technical support (techsupport@lgcgroup.com) if you require any guidance.\u003c\/p\u003e\u003cp\u003ePlease note that manually optimised protocols may require further optimisation when transferring to an automated platform.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe sbeadex Lightning Nucleic Acid Purification Kit comes as a modular system to tailor your optimal kit. Choose from two versions of sbeadex Lightning, Core Kit A and Core Kit B.\u003c\/p\u003e\u003cp\u003eEach kit contains:\u003c\/p\u003e\u003cp\u003esbeadex Lightning binding buffer (LP in Kit A and LU in Kit B)\u003cbr\u003esbeadex particles suspension\u003cbr\u003eelution buffer (AMP)\u003c\/p\u003e\u003cp\u003eCore kits can be complemented with a range of individual components (e.g. lysis buffers, Protease K solution, debris capture beads) that can be purchased separately. Just buy what you need to establish your optimal purification method!\u003c\/p\u003e\u003cp\u003eWe highly recommend starting with our comprehensive starter kit to evaluate performance and optimise workflow before progressing to one of our core kits and lysis buffers, especially for those unfamiliar with our product line.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003eautomation sbeadex Lightning Hamilton\u003c\/p\u003e\u003cp\u003eAdditional resources\u003c\/p\u003e\u003cp\u003eEXTERNAL LINK\u003c\/p\u003e\u003cp\u003esbeadex Lightning competitor comparision app note\u003c\/p\u003e\u003cp\u003eEXTERNAL LINK\u003c\/p\u003e\u003cp\u003esbeadex Lightning automation Dynamic Devices Lynx app note\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eApplication data\u003c\/h2\u003e\n\u003cp\u003e- DNA purification for many downstream applications such as PCR\/qPCR, RT-PCR or NGS\u003c\/p\u003e\u003cp\u003e- Applicable to a wide range of sample types including plant and animal tissues\u003c\/p\u003e\u003cp\u003ePlease find a complete list of already tested plant and animal samples with a recommendation of the appropriate Core Kit and Lysis buffers\/conditions here.\u003c\/p\u003e\u003cp\u003esbeadex Lightning has been successfully tested against popular plant DNA purification kits from market-leading competitors, showing comparable or even better DNA purities and yields as well as a clear reduction of protocol steps and time – in addition to significant savings regarding consumption of plastic consumables and amount of liquid waste. Moreover, DNA isolated using sbeadex Lightning also showed a very good integrity compared to DNA isolated using those competitor kits.\u003c\/p\u003e\u003cp\u003eFor more details please download the sbeadex Lightning app note.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBXLCK_MSDS-EN-Binding-buffer-LP\"\u003eMSDS EN Binding buffer LP\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBXLCK_MSDS-EN-Binding-buffer-LU\"\u003eMSDS EN Binding buffer LU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBXLCK_MSDS-EN-Elutions-Buffer-AMP\"\u003eMSDS EN Elutions Buffer AMP\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_SBXLCK_MSDS-EN-sbeadex-particle-suspension\"\u003eMSDS EN sbeadex particle suspension\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/protocol_SBXLCK_sbeadex-Lightning-protocol-for-plant-and-livestock-tissue-DNA\"\u003esbeadex Lightning protocol for plant and livestock tissue DNA\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_SBXLCK_sbeadex-Lightning-Hamilton-manual\"\u003esbeadex Lightning Hamilton manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_SBXLCK_sbeadex-Lightning-Starter-Kit-quick-guide\"\u003esbeadex Lightning Starter Kit quick guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_SBXLCK_sbeadex-Lightning-buffer-recommendations-guide\"\u003esbeadex Lightning buffer recommendations guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_SBXLCK_sbeadex-Lightning-manual\"\u003esbeadex Lightning manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/data-sheet_SBXLCK_sbeadex-Lightning-data-sheet\"\u003esbeadex Lightning data sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_SBXLCK_automation-sbeadex-Lightning-Hamilton\"\u003eautomation sbeadex Lightning Hamilton\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchassets.biosearchtech.com\/assetsv6\/guide_sbeadex-Lightning-buffer-recommendations.pdf\"\u003ehere\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"NAP40-030-01","offer_id":45986466103375,"sku":"NAP40-030-01","price":1695.0,"currency_code":"ILS","in_stock":true},{"title":"NAP40-030-02","offer_id":45986466136143,"sku":"NAP40-030-02","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"000 purifications)","offer_id":45986466168911,"sku":"NAP40-030-03","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"NAP40-031-01","offer_id":45986466201679,"sku":"NAP40-031-01","price":1494.0,"currency_code":"ILS","in_stock":true},{"title":"NAP40-031-02","offer_id":45986466234447,"sku":"NAP40-031-02","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"000 purifications) (NAP40-031-03)","offer_id":45986466267215,"sku":"NAP40-031-03","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/sbeadex-Lightning-Nucleic-Acid-Purification-Kit_4e3d969d-b331-49b3-9bcd-cb93e6f011e8.jpg?v=1785956575","url":"https:\/\/www.danyel.co.il\/products\/sbeadex-lightning-nucleic-acid-purification-kit-modular-sbxlck","provider":"danyel biotech","version":"1.0","type":"link"}