{"product_id":"sars-cov-2-neutralization-antibody-detection-elisa-kit-tbs3240","title":"SARS-CoV-2 Neutralization Antibody Detection ELISA Kit","description":"\u003cdiv class=\"wpb-content-wrapper\"\u003e\n\u003cdiv class=\"vc_row wpb_row row top-row\"\u003e\u003cdiv class=\"vc_column_container col-md-12\"\u003e\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\t\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\t\t\u003cdiv class=\"wpb_wrapper\"\u003e\n\t\t\t\u003cp\u003eThe ELISA assay is based on the competitive principle. Each well of the supplied microtiter plate has been pre-coated with recombinant SARS-CoV-2 Spike RBD-hFc protein. When HRP conjugated recombinant human ACE2 protein is added simultaneously to wells along with diluted human serum\/plasma samples, immobilized RBD will bind human ACE2 protein. The binding of RBD to human ACE2 protein is dose-dependently inhibited by the presence of anti-SARS-CoV-2 human neutralization antibodies in serum samples. Following the sample incubation step, unbound ACE2-HRP conjugate is washed away. A Positive Control is a known human antibody (IgG1 subtype) that is specific for SARS-CoV-2 spike protein with a binding epitope for RBD binding site. The positive control antibody is validated by an established pseudovirus infection of cultured human cells for proven neutralization function. Human IgG1 isotype control is used as a Negative Control sample. The absorbance of the unknown sample can then be compared to the Positive and Negative controls to determine the absence or presence of anti-SARS-CoV-2 human neutralization antibodies. In the competition assay, the greater the amount of anti-SARS CoV-2 human neutralization antibodies in the sample, the lower the color development and optical density reading.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity: \u003c\/strong\u003eThis kit is for the detection of Human anti-SARS-CoV-2 neutralization antibodies.\u003c\/p\u003e\n\u003cdiv dir=\"ltr\"\u003e\u003c\/div\u003e\n\u003cdiv dir=\"ltr\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eMain Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple:\u003c\/strong\u003e The sample and HRP detection combined into one step\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast:\u003c\/strong\u003e Just 1-hour incubation and 1step washing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e detection of 7.0 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Specifically detecting human ant-SARS-CoV-2 neutralization antibodies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample Types: \u003c\/strong\u003eThis kit is recommended for use with human serum\/plasma.  Use with other sample types like SARS-CoV-2 immunized animal serum is possible but not validated in this testing kit.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range:\u003c\/strong\u003e 10 to 500 ng\/ml using positive control antibody 8A5\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e Typically, about 7.0 ng\/mL with IC50 at approximately 50.0 ng\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePerformance: \u003c\/strong\u003eIntra-Assay CV (\u0026lt; 10.0%); Inter-Assay CV (\u0026lt; 15.0%)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3240--\u003e\u003cimg class=\"aligncenter wp-image-6925 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/01\/one-step_fasterELISAGraphic.png\" alt=\"\" width=\"539\" height=\"120\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAssay Protocol\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"t pg-6m0 pg-6x0 pg-6h4 pg-6y3 pg-6ff1 pg-6fs1 pg-6fc1 pg-6sc0 pg-6ls0 pg-6ws0\"\u003e\u003cstrong\u003e\u003cimg class=\"aligncenter wp-image-6929 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/01\/Picture1.png\" alt=\"\" width=\"641\" height=\"327\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"t pg-6m0 pg-6x0 pg-6h4 pg-6y3 pg-6ff1 pg-6fs1 pg-6fc1 pg-6sc0 pg-6ls0 pg-6ws0\"\u003eThe following standard curve is an example only and should not be used to calculate results for tested samples. A new standard curve must be generated for each set of samples tested.\u003c\/div\u003e\n\u003cp\u003e\u003cimg class=\"size-medium wp-image-6926 aligncenter\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/01\/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20210210123446-300x220.png\" alt=\"\" width=\"300\" height=\"220\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"t pg-6m0 pg-6x0 pg-6h4 pg-6y6 pg-6ff1 pg-6fs1 pg-6fc1 pg-6sc0 pg-6ls0 pg-6ws0\"\u003eSerum samples from SARS-CoV-2 recovered patients (n=10) and healthy individuals (n=10) were tested using Tribioscience SARS-CoV-2 neutralization antibody detection ELISA kit. The serum samples from recovered patients (\u0026gt;90%) have a detectable level of neutralization antibody (average % of inhibition is about 61%). In contrast, the serum samples from healthy individuals do not have any obvious presence of neutralization antibody (average % of inhibition is 27%).\u003c\/div\u003e\n\u003cp\u003e\u003cimg class=\"size-medium wp-image-6927 aligncenter lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/01\/2-300x203.png\" alt=\"\" width=\"300\" height=\"203\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\n\t\t\u003c\/div\u003e\n\t\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/01\/TBS3240_SARS-CoV-2-Neutralization-Antibody-ELISA-Kit-Manual-V2-02.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/01\/TBS3240_SARS-CoV-2-Neutralization-Antibody_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986382577743,"sku":"TBS3240","price":2228.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3204.jpg?v=1782809960","url":"https:\/\/www.danyel.co.il\/products\/sars-cov-2-neutralization-antibody-detection-elisa-kit-tbs3240","provider":"danyel biotech","version":"1.0","type":"link"}