{"product_id":"rprotein-a-sepharose-fast-flow-antibody-purification-resin-17127903","title":"rProtein A Sepharose™ Fast Flow antibody purification resin","description":"\u003cp\u003erProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor monoclonal and polyclonal antibody purification at both laboratory and process scale.\u003c\/li\u003e\n\u003cli\u003eWell established among Protein A resins as excellent for monoclonal antibody (mAb) processes.\u003c\/li\u003e\n\u003cli\u003eFree from animal-derived components.\u003c\/li\u003e\n\u003cli\u003eHydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.\u003c\/li\u003e\n\u003cli\u003eFulfills industrial demands for security of supply, robust performance, and regulatory support.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMonoclonal and polyclonal antibody purification\u003c\/p\u003e\u003cp\u003erProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.\u003c\/p\u003e\u003cp\u003eProtein A resin characteristics\u003c\/p\u003e\u003cp\u003eThe recombinant protein is produced in \u003ci\u003eE. coli\u003c\/i\u003e and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.\u003c\/p\u003e\u003cp\u003eStreamlined scale up\u003c\/p\u003e\u003cp\u003eScale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eCytiva's protein A resins are used in a variety of applications, including:\n\u003c\/p\u003e\u003cul\u003e\u003cli\u003eMolecular characterization of an \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1782203\/\"\u003earthritogenic collagen peptide interacting with I-Ar\u003c\/a\u003e\n\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eCompatible empty columns – research scale\u003c\/p\u003e\u003cp\u003eCytiva's solutions include a variety of empty columns for packing. rProtein A Sepharose resin is compatible for use with HiScale, Tricorn, and XK  columns.\u003c\/p\u003e\u003cp\u003eCompatible empty columns – bioprocess scale\u003c\/p\u003e\u003cp\u003eFor process-scale applications, Cytiva recommends AxiChrom columns. rProtein A Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.\u003c\/p\u003e\u003cp\u003eConvenient prepacked columns – research scale\u003c\/p\u003e\u003cp\u003erProtein A Sepharose Fast Flow is also available in convenient prepacked formats:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003erProtein A GraviTrap,  for use with gravity flow\u003c\/li\u003e\n\u003cli\u003eHiTrap rProtein A FF, for use with a syringe, pump, or chromatography system\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eConvenient prepacked columns – bioprocess scale\u003c\/p\u003e\u003cp\u003ePrepacked ReadyToProcess columns are available on request, eliminating the need for column packing and cleaning validation.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11466-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-12959-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"5 mL","offer_id":45982279270479,"sku":"17127901","price":3749.0,"currency_code":"ILS","in_stock":true},{"title":"25 mL","offer_id":45982279303247,"sku":"17127902","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"200 mL","offer_id":45982279336015,"sku":"17127903","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-5558-original-png_e2ade563-4c87-43fb-8b90-ec04a8e8ee1b.png?v=1785959811","url":"https:\/\/www.danyel.co.il\/products\/rprotein-a-sepharose-fast-flow-antibody-purification-resin-17127903","provider":"danyel biotech","version":"1.0","type":"link"}