{"product_id":"rprotein-a-and-protein-g-sepharose-4-ff-in-gravitrap-columns-28985256","title":"rProtein A and Protein G Sepharose™ 4 FF in GraviTrap columns","description":"\u003cp\u003erProtein A Protein G GraviTrap contains ten columns with 1 mL 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow resins.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor general-purpose purification of monoclonal and polyclonal antibodies.\u003c\/li\u003e\n\u003cli\u003eSuitable when the affinity for protein A and protein G is not known.\u003c\/li\u003e\n\u003cli\u003eGraviTrap prepacked gravity flow columns enable simple one-step purification of proteins in 30 min without the need for equipment.\u003c\/li\u003e\n\u003cli\u003eHigh purity: \u0026gt; 95% in one step.\u003c\/li\u003e\n\u003cli\u003eHigh protein recovery (typically 70% to 80%) due to high binding capacity of ~ 35 mg IgG\/mL resin.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFast and simple antibody purification\u003c\/p\u003e\u003cp\u003eThis column can be used for manual purification of polyclonal and monoclonal antibodies from cell culture supernatant and biological fluids. It is particularly suitable when the affinity for protein A and protein G is not known. The gravity flow column relies on gravity and is reusable up to five times, so no additional instruments are needed for this protocol.\u003c\/p\u003e\u003cp\u003eOther Cytiva products for antibody affinity chromatography\u003c\/p\u003e\u003cp\u003eCytiva's solutions include a variety of resins, prepacked columns, and other formats for purifying antibodies and antibody fragments.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-15854-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28987231.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":45982034460751,"sku":"28985256","price":6120.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-30257-original-png_43facd57-de55-402f-a67e-4c4c1c7e719a.png?v=1785957220","url":"https:\/\/www.danyel.co.il\/products\/rprotein-a-and-protein-g-sepharose-4-ff-in-gravitrap-columns-28985256","provider":"danyel biotech","version":"1.0","type":"link"}