{"product_id":"rnase-free-dnase-i-mbenz-001","title":"RNase-Free DNase I","description":"\u003cp\u003eRNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation. It efficiently hydrolyses dsDNA and ssDNA into a mixture of short oligonucleotides and mononucleotides.\u003c\/p\u003e\u003cp\u003eApplications\u003cbr\u003eElimination of template DNA following in vitro synthesis of RNA with T7, SP6, or T3 RNA polymerase.\u003cbr\u003eLabeling of DNA by nick translation, in combination with Klenow or other DNA polymerases.\u003cbr\u003eTreatment of RNA prior to RT-PCR. 1\u003cbr\u003eCharacterisation of DNA-protein interactions by DNase I footprinting. 2,3\u003c\/p\u003e\u003cp\u003eFigure 1. DNA removal from in vitro transcription reactions using RNase-Free DNase I. A linearised DNA template was transcribed using T7 RNA polymerase according to standard in vitro transcription conditions. Lane 1, kb ladder; Lane 2, DNA control; Lane 3, transcription mixture; Lane 4, transcription mixture treated with 1 MBU of RNase-Free DNase I for 15 minutes at 37 °C.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eKienzle, N. et al. (1996) BioTechniques 20, 612.\u003cbr\u003eSambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.) Cold Spring Harbor Laboratory Press, New York.\u003cbr\u003eGalas, D.J. and Schmitz, A. (1978) Nucleic Acids Res. 5, 3157.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDegrade dsDNA and ssDNA without damaging important RNA in your sample or reaction\u003c\/li\u003e\n\u003cli\u003eDigest away unwanted DNA without digesting RNA\u003c\/li\u003e\n\u003cli\u003eUse in a variety of applications such as in vitro transcription and RT-PCR\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One Molecular Biology Unit (MBU) of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutes at 37 °C under standard assay conditions.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, and 10 mM MgCl2.\u003c\/p\u003e\u003cp\u003eDNase I 10X Reaction Buffer: 100 mM Tris-HCl (pH 7.5), 25 mM MgCl2, and 5 mM CaCl2.\u003c\/p\u003e\u003cp\u003eQuality Control: No degradation of 1 µg of a synthetic RNA transcript is detected by agarose gel electrophoresis following incubation with 10 U of RNase-Free DNase I at 37 °C for 1 hour.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eIncludes 10X Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_MBENZ-001_D9905K-D9910K-RNase-Free-DNase-I\"\u003eD9905K D9910K RNase Free DNase I\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_D9905K_RNase-Free-DNase-I\"\u003eRNase Free DNase I\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_MBENZ-001_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986440020047,"sku":"D9905K","price":1206.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RNase-Free-DNase-I_b76b48bc-4447-4704-9ae5-7ab79e6428d4.jpg?v=1785956214","url":"https:\/\/www.danyel.co.il\/products\/rnase-free-dnase-i-mbenz-001","provider":"danyel biotech","version":"1.0","type":"link"}