{"product_id":"rna-delivery-lnp-kit-1002569","title":"RNA delivery LNP kit","description":"\u003cp\u003eThe RNA delivery LNP kit for the NanoAssemblr™ Ignite™ and Ignite+™ systems offers ready–to–use ionizable lipid mix and buffers to fast-track payload screening and RNA drug delivery validation, progressing lead candidates quickly through preclinical studies accelerating clinical evaluation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePreoptimized ionizable lipid mix for RNA-LNP delivery to accelerate lead candidate selection\u003c\/li\u003e\n\u003cli\u003eRapid and easy prep of high-quality, stable LNPs using prepared reagents and protocols\u003c\/li\u003e\n\u003cli\u003eEasily scalable on the NanoAssemblr™ system platform\u003c\/li\u003e\n\u003cli\u003eIncludes in vitro protein expression and in vivo immunogenicity proof-of-concept data for mRNA vaccine applications, validated for reproducible, high-quality LNP production and stability, up to clinical and commercial nanomedicine manufacturing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e*LNP composition in this research-use-only kit includes an ionizable lipid excipient that can be licensed for future clinical evaluation.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eStreamline payload screening and lead candidate optimization\u003c\/p\u003e\n\n\u003cp\u003eOff-the-shelf reagent kits offer easy screening of RNA payloads and lead candidate optimization for physicochemical attributes and potency to fast-track lead candidate selection for RNA drug delivery.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/7VmaLhmKSXOY1rW62o4UJw-original-png?v=a458877c\" style=\"max-width: 700px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eGraphs above show (left) the LNP size and polydispersity (PDI), and (right) the encapsulation efficiency of LNPs, prepared using the RNA delivery LNP kit with both mRNA and self-amplifying RNA encoding for different antigens.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/xeBJC5elSDq2dJLAF_ShFA-original?v=04fc1acf\" style=\"max-width: 600px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eLNPs were prepared using the RNA delivery LNP kit and mRNA encoding for haemagglutinin (HA), a surface protein on the H1N1 virus. LNPs were used to immunize BALB\/c mice with a primary and booster dose of 2 μg RNA on days 0 and 28 of the immunization schedule. The immunogenicity of LNPs is shown in the graph above, showing the HA inhibition titers using serum sampled on days 21 and 42.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eQuality RNA-LNP formulations\u003c\/p\u003e\n\n\u003cp\u003eThe RNA delivery LNP kit consistently produces quality LNPs that are highly effective in protecting encapsulated nucleic acids, maintaining critical quality attributes (CQAs) and potency over time.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/_imLlUV-RWOYqIHkMliSgQ-original?v=488c4998\" style=\"max-width: 850px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eStorage stability and CQA consistency of LNPs produced with the RNA delivery LNP kit, prepared with cryopreservation buffer, over 1 week of storage under refrigerated conditions and 6 weeks of storage at -80°C.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/8xEaYUR8TkK9ixYvNxRLsw-original-png?v=20640fd0\" style=\"max-width: 850px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eIn vitro\u003c\/em\u003e potency of lead candidate LNPs encapsulating (A) eGFP mRNA or (B) eGFP saRNA in HEK-293 cells over 1 week of storage under refrigerated conditions and 6 weeks of storage at -80°C.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eScalable NxGen™ technology\u003c\/p\u003e\n\n\u003cp\u003eGenomic medicine success starts at the discovery phase, by validating RNA drug delivery with ionizable lipid compositions and LNP technologies at a small scale. This allows for consistent LNP formulation using the NanoAssemblr™ NxGen™ platform for clinical evaluation and commercial production.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/AeegOA3SRmiUMlGmnBZBUA-original-png?v=6ac90a53\" style=\"max-width: 700px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eImmunogenicity of saRNA-LNPs prepared using NanoAssemblr™ NxGen™ technology at different scales, showing the log-transformed SARS-CoV-2 spike protein specific IgG titers in serum of BALB\/c mice treated with lead candidate saRNA-LNPs.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eKit components\u003c\/p\u003e\n\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable class=\"table table-bordered\"\u003e\n\t\u003cthead\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003cth class=\"-font--body\"\u003e \u003c\/th\u003e\n\t\t\t\u003cth class=\"-font--body\"\u003eRNA delivery LNP kit, 3 mL\u003cbr\u003e\n\t\t\t1002569\u003c\/th\u003e\n\t\t\t\u003cth class=\"-font--body\"\u003eRNA delivery LNP kit, 6 mL\u003cbr\u003e\n\t\t\t1002471\u003c\/th\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/thead\u003e\n\t\u003ctbody\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eLipid mix\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e3 mL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e6 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eFormulation buffer\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e6 mL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e6 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eDilution buffer\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e100 mL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e100 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eCryopreservation buffer\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e12 mL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e12 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29798795_fr.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"Ignite, Ignite+ (1002569)","offer_id":45982350049359,"sku":"1002569","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ignite, Ignite+ (1002471)","offer_id":45982350082127,"sku":"1002471","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E5vcyaJGTdKW1hx-AhUyzA-original-png_8c5166a2-9771-44da-b47d-6c3d10d85aaa.png?v=1785960393","url":"https:\/\/www.danyel.co.il\/products\/rna-delivery-lnp-kit-1002569","provider":"danyel biotech","version":"1.0","type":"link"}