{"product_id":"rna-5-polyphosphatase-rp8092h","title":"RNA 5' Polyphosphatase","description":"\u003cp\u003eRNA 5´ Polyphosphatase* is a Mg2+-independent phosphohydrolase discovered and characterised by Epicentre scientists. The enzyme sequentially removes the γ and β phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts):\u003cbr\u003e5´ pppN—OH 3´ to ; 5´ pN—OH 3´ + 2 Pi\u003c\/p\u003e\u003cp\u003eRNAs with a 5´-diphosphorylated end are also converted to 5´-monophosphorylated RNA by RNA 5´ Polyphosphatase:\u003cbr\u003e5´ ppN—OH 3´ to ; 5´ pN—OH 3´ + Pi\u003c\/p\u003e\u003cp\u003eRNA Polyphosphatase has no activity on RNA with a 5´ cap (e.g., 5´ m7GpppN—OH 3´), or a 5´-monophosphorylated end (5´ pN—OH 3´). However, both NTPs and dNTPs are substrates for the enzyme, yielding the corresponding NMPs and dNMPs + inorganic phosphate: (d)NTP to ; (d)NMP + 2Pi\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eConversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase.\u003cbr\u003eAnalysis of 5´-end structure of RNA.\u003cbr\u003ePreparation of substrate RNA molecules for subsequent degradation using the Epicentre Terminator™ 5´-Phosphate-Dependent Exonuclease.\u003c\/p\u003e\u003cp\u003e*Covered by issued and\/or pending patents.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDephosphorylates 5´-triphosphorylated RNA, such as primary RNA transcripts, but does not dephosphorylate 5´-capped mRNA\u003c\/li\u003e\n\u003cli\u003eSpecific: Removes the γ and β phosphates from 5´-triphosphorylated and the β phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5´-capped RNA\u003c\/li\u003e\n\u003cli\u003eHeat Inactivated: Heat at 65 °C for 20 minutes to kill enzyme activity\u003c\/li\u003e\n\u003cli\u003eFlexible: Use for various applications such as 5´-RNA ligation-tagging using T4 RNA Ligase, analysis of 5´-end structure of RNA, and preparation of substrate RNAs for subsequent degradation with Terminator 5´-Phosphate-Dependent Exonuclease\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of RNA 5´ Polyphosphatase releases 1 nmol of inorganic phosphate from ATP in 1 hour at 37 °C under standard assay conditions.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 0.05 M Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003e10X Reaction Buffer: 0.5 M HEPES-KOH (pH 7.5), 1 M NaCl, 10 mM EDTA, 1% β-mercaptoethanol, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003eInactivation\/Inhibitors: RNA 5´ Polyphosphatase is inhibited ~50% by 100 mM of inorganic phosphate (Pi) using ATP as a substrate.\u003c\/p\u003e\u003cp\u003eQuality Control: RNA 5´ Polyphosphatase is free of detectable DNA exo- and endonuclease, and RNase activities.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eIncludes 10X Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_RP8092H_RNA-5-Polyphosphatase\"\u003eRNA 5 Polyphosphatase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_RP8092H_RNA-5-Polyphosphatase-User-Manual\"\u003eRNA 5 Polyphosphatase User Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_RP8092H_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986473214031,"sku":"RP8092H","price":998.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RNA-5_2527-Polyphosphatase_966f7251-6d50-4175-8dd8-de3f3fd48d22.jpg?v=1785956813","url":"https:\/\/www.danyel.co.il\/products\/rna-5-polyphosphatase-rp8092h","provider":"danyel biotech","version":"1.0","type":"link"}