{"product_id":"ripa-buffer-tbs5017","title":"RIPA Buffer","description":"\u003cp\u003eRadioImmunoPrecipitation Assay (RIPA) Buffer is used to lyse cells and tissues under non-denaturing conditions.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eDirections for Use:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAliquotting of RIPA buffer is recommended if many small experiments are to be performed. For longer periods of time, the buffer should be stored at –20°C. If buffer will be continually used, it is recommended that the buffer be kept at 4°C for 1-2 weeks.\u003c\/li\u003e\n\u003cli\u003eThaw RIPA buffer at room temperature, mixing end-over-end.\u003c\/li\u003e\n\u003cli\u003eChill RIPA buffer on ice and add PMSF (final 1 mM) just prior to use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSupplied as a ready to use solution, 50mL. 100mM PMSF, 0.5mL.\u003cbr\u003e\nStore at –20℃. For short-term storage (1-2 weeks), RIPA Buffer can be stored at 4°C.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS5017--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS5017_RIPA_buffer_111215-2.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS5017_RIPA_buffer_MSDS-2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003e1. Pan, B., Zhan, X., \u0026amp; Li, J. (2022). MicroRNA-574 Impacts Granulosa Cell Estradiol Production \u003cem\u003evia\u003c\/em\u003e Targeting TIMP3 and ERK1\/2 Signaling Pathway. \u003cem\u003eFrontiers in endocrinology\u003c\/em\u003e, \u003cem\u003e13\u003c\/em\u003e, 852127. \u003ca href=\"https:\/\/doi.org\/10.3389\/fendo.2022.852127\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.3389\/fendo.2022.852127\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e2. Pan, B., Liu, C., Zhan, X., \u0026amp; Li, J. (2021). Protegrin-1 Regulates Porcine Granulosa Cell Proliferation \u003cem\u003evia\u003c\/em\u003e the EGFR-ERK1\/2\/p38 Signaling Pathway \u003cem\u003ein vitro\u003c\/em\u003e. \u003cem\u003eFrontiers in physiology\u003c\/em\u003e, \u003cem\u003e12\u003c\/em\u003e, 673777. \u003ca href=\"https:\/\/doi.org\/10.3389\/fphys.2021.673777\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.3389\/fphys.2021.673777\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986334638159,"sku":"TBS5017","price":178.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS5017-50.jpg?v=1782808834","url":"https:\/\/www.danyel.co.il\/products\/ripa-buffer-tbs5017","provider":"danyel biotech","version":"1.0","type":"link"}