{"product_id":"ready-to-go-you-prime-first-strand-beads-27926401","title":"Ready-To-Go You-Prime First-Strand Beads","description":"\u003cp\u003eThe kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 ml tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice for RT-PCR.\u003c\/li\u003e\n\u003cli\u003ePredispensed, ambient-temperature-stable, single-dose reaction beads minimize pipetting errors, avoid cross-contamination, and ensure optimal performance.\u003c\/li\u003e\n\u003cli\u003eFirst-strand reaction beads contain no primer, thus allowing users to select a first-strand primer of choice.\u003c\/li\u003e\n\u003cli\u003eHighly suitable for research applications that use PCR to detect and quantify eukaryotic RNA from a variety of samples.\u003c\/li\u003e\n\u003cli\u003eReaction beads are function-tested for first-strand synthesis of cDNA up to 7.5 kb and in RT-PCR from blood samples.\u003c\/li\u003e\n\u003cli\u003eCompleted reactions (33 µl final volume) can be used directly in PCR after adding water, Taq DNA polymerase, and primers; first-strand cDNA can also be used as a template for traditional Gubler-Hoffman second-strand cDNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach kit contains predispensed first-strand reaction mixes which are treated by a special process turning each one into two small beads, stable at ambient temperatures. Each first-strand reaction is quickly reconstituted when mixed with an aqueous solution. Thus, with Ready-To-Go You-Prime First-Strand Beads, preparation and setup are convenient. The beads are reconstituted with the denatured RNA sample, primer is added, and the reaction is placed at 37°C for 60 minutes. The completed first-strand reaction is then available for PCR amplification or second-strand cDNA synthesis.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/27926401PL.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"50 reactions","offer_id":45982292639823,"sku":"27926401","price":3186.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-9523-original-png_2ddda3f4-14d6-4110-9229-c485a4a914a4.png?v=1785960038","url":"https:\/\/www.danyel.co.il\/products\/ready-to-go-you-prime-first-strand-beads-27926401","provider":"danyel biotech","version":"1.0","type":"link"}