{"product_id":"ready-lyse-lysozyme-solution-r1810","title":"Ready-Lyse Lysozyme Solution","description":"\u003cp\u003eReady-Lyse Lysozyme Solution is a stabilised lysozyme preparation for general bacterial lysis of Gram-positive or Gram-negative bacteria. Ready-Lyse’s specific activity is over 200 times higher than the commonly used egg-white lysozyme. This improves bacterial lysis and uses less enzyme, virtually eliminating losses due to nonspecific binding in nucleic acid purifications.\u003c\/p\u003e\u003cp\u003eComplete digestion should occur within 15 minutes at room temperature. Following lysis, the lysate can be treated according to standard protocols for the purification of nucleic acids or proteins.\u003c\/p\u003e\u003cp\u003eFor difficult to lyse bacteria, Ready-Lyse Lysozyme Solution can be an additional lysis step when paired with our QuickExtract™ DNA Extraction Solution or MasterPure™ Gram Positive DNA Purification Kit, for DNA extraction. QuickExtract is formulated for bacterial DNA extractions, making it an optimal solution for DNA extraction from gram +\/- bacteria, and our MasterPure Kit provides all of the reagents necessary to purify DNA from Gram-positive bacteria. Both QuickExtract and MasterPure can be used alone or with Ready-Lyse Lysozyme Solution.\u003c\/p\u003e\u003cp\u003eThe process is simple. Just add Ready-Lyse and QuickExtract to a bacteria sample and incubate at room temperature for 15 minutes. To kill remaining viable bacteria, the samples can be heated to 80 °C for 2 minutes.\u003c\/p\u003e\u003cp\u003eFigure 1. Decreased loss of DNA with Ready-Lyse Lysozyme Solution compared to egg-white lysozyme.\u003c\/p\u003e\u003cp\u003epHC79 cosmid DNA (500 μg\/mL) was incubated for 15 minutes at 22 °C with either 5 μg (30 KU)\/mL of Ready-Lyse Lysozyme (RL), 500 μg\/mL of egg-white lysozyme (EW) or no lysozyme (C) in conditions typically used for lysis of E. coli (25 mM Tris [pH 8.0], 10 mM EDTA). The solutions were then microcentrifuged for 10 minutes. The supernatants were removed and the pellets were resuspended in TE buffer containing 0.1% SDS. Supernatants (lanes 1-3) and pellets (lanes 5-7) were then analysed by electrophoresis in a 0.8% agarose gel.\u003c\/p\u003e\u003cp\u003eThe DNA extracted can be used for PCR, pulse-field gel electrophoresis (PFGE), restriction digestion, or optical mapping.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHighly Active: 200X more active than egg-white lysozyme and optimally active at a lower pH of 6.5-7.5 than the standard pH 9.\u003c\/li\u003e\n\u003cli\u003eEasy: No sample agitation or heat generation necessary during lysis which helps preserve protein function\u003c\/li\u003e\n\u003cli\u003eFlexible: Usage volumes are easily adjusted based on the scale of the experiment\u003c\/li\u003e\n\u003cli\u003eStable: stable at -20 °C, eliminating the need to prepare a fresh solution for each use\u003c\/li\u003e\n\u003cli\u003eAccurate: virtually no nonspecific binding and significantly less precipitation of DNA compared to egg-white lysozyme\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of Ready-Lyse Lysozyme produces a decrease in A 350 of 0.001 per minute at 23 °C with a 0.5 mg\/mL suspension of lyophilised E. coli K802 cells in 50 mM Tris-HCl (pH 7.5).\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eReady-Lyse Lysozyme Solution a ready-to-use solution supplied in quantities of 4 or 10 × 106 units.\u003c\/p\u003e\u003cp\u003eExpert Recommendation: For difficult to lyse bacteria, Ready-Lyse  Lysozyme Solution can be an additional lysis step when paired with our QuickExtract™ DNA Extraction Solution or MasterPure™ Gram Positive DNA Purification Kit, to help extract PCR-ready DNA from gram +\/- bacteria or fungi. Both QuickExtract and MasterPure can be used alone or with Ready-Lyse Lysozyme Solution.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_R1810_R1810M-Ready-Lyse-Lysozyme-Solution\"\u003eR1810M Ready Lyse Lysozyme Solution\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_R1810_Ready-Lyse%20Lysozyme%20Solution\"\u003eReady Lyse Lysozyme Solution\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_R1810_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"4,000,000 U","offer_id":45986472886351,"sku":"R1804M","price":1977.0,"currency_code":"ILS","in_stock":true},{"title":"10,000,000 U","offer_id":45986472919119,"sku":"R1810M","price":3765.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Ready-Lyse-Lysozyme-Solution_e6d6f4a6-09e8-420a-8bb6-2e489780e32a.jpg?v=1785956793","url":"https:\/\/www.danyel.co.il\/products\/ready-lyse-lysozyme-solution-r1810","provider":"danyel biotech","version":"1.0","type":"link"}