{"product_id":"qiagen-forenseq-imagen-kit-v16000189","title":"Verogen ForenSeq Imagen Kit","description":"\u003cp\u003eThe Verogen ForenSeq Imagen kit is a library preparation kit offering two different primer mixes that allow the user to evaluate phenotypic SNPs with or without biogeographical ancestry. The ForenSeq Imagen workflow is a fully validated DNA-to-data workflow specifically designed for forensic genomics applications. The kit relies on established, peer-reviewed SNP marker sets that have been identified and tested by the forensic community.\u003c\/p\u003e\n\u003cp\u003eThe Verogen ForenSeq Imagen Kit is an optimized solution for operational forensic labs considering implementing an NGS-based lead generation workflow.\u003c\/p\u003e\u003cp\u003eIncludes the required reagents to process up to 96 samples simultaneously for phenotypic and biogeographical ancestry information to generate comprehensive DNA profiles\u003c\/p\u003e\u003ch2\u003eFeatures\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eEvaluation of phenotypic SNPs with or without biogeographical ancestry\u003c\/li\u003e\n\u003cli\u003eWorkflow tailored to integrate in operational forensic laboratories\u003c\/li\u003e\n\u003cli\u003eInteroperable and scalable with preconfigured reports for upload to third party tools\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003ePerformance\u003c\/h2\u003e\u003cp\u003eThe ForenSeq Imagen Kit offers the following features:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eA concentrated primer mix for increased sample input volume\u003c\/li\u003e\n\u003cli\u003eSimultaneous preparation of up to 96 libraries in one plate; each library is a collection of amplified DNA fragments from one sample\u003c\/li\u003e\n\u003cli\u003eAssay designed to maximize DNA profiles with degraded and low-input samples by generating short amplicons with Unique Dual Indices (UDIs)\u003c\/li\u003e\n\u003cli\u003eBuffer system to support inhibited forensic samples\u003c\/li\u003e\n\u003cli\u003eTwo primer mixes to generate insights based on existing and emerging regional regulation \n\u003cul\u003e\n\u003cli\u003eDPME – Amplifies SNP targets for the prediction of skin, hair and eye color, also referred to as external visible characteristics (EVC)\u003c\/li\u003e\n\u003cli\u003eDPMF (Geo) – Amplifies SNP targets for the prediction of EVCs in DPME and biogeographical ancestry (BGA) markers\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBoth primer sets encompass 41 SNPs for the phenotypic estimations that include 22 SNPs for the estimation of hair color, 6 SNPs for the estimation of eye color, 36 markers for the estimation of skin color, with some SNPs common to more than one phenotype. Both primer sets encompass 14 Y-SNPs to enable robust estimation of biological sex. In addition, 56 SNPs for the estimation of biogeographic ancestry are included in one of the primer sets. Labs have the flexibility to use either of the two primer sets based on the regulatory approaches associated with their geography.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeature\u003c\/td\u003e\n\u003ctd\u003eDetails\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eExtracted gDNA from a range of common forensic sample types:\u003cbr\u003ebuccal swab lysate, bones, blood, teeth and FTA card \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended input for gDNA\u003c\/td\u003e\n\u003ctd\u003e32 pg–1 ng per sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended input for FTA card punch\u003c\/td\u003e\n\u003ctd\u003e1.2 mm per sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended multiplexing capacity\u003c\/td\u003e\n\u003ctd\u003e8–96 libraries per run\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNumber of primer mixes\u003c\/td\u003e\n\u003ctd\u003e2: DPME and DPMF (Geo)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrimer mix content\u003c\/td\u003e\n\u003ctd\u003eDPME: 22 hair SNPs, 6 eye color SNPs, 36 skin color SNPs \u003cbr\u003eand 14 Y-SNPs (total of 55 SNPs)\u003cbr\u003eDPMF (Geo): 22 hair SNPs, 6 eye color SNPs, 36 skin color SNPs, \u003cbr\u003e56 BGA SNPs and 14 Y-SNPs (total of 111 SNPs)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShort amplicon detection\u003c\/td\u003e\n\u003ctd\u003eHigh concentration of markers \u0026lt;150 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal library prep time\u003c\/td\u003e\n\u003ctd\u003e7 hours and 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHands-on time\u003c\/td\u003e\n\u003ctd\u003e1 hour and 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal sequencing time\u003c\/td\u003e\n\u003ctd\u003e~18 hours with the Verogen MiSeq FGx Reagent Micro Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003ch2\u003ePrinciple\u003c\/h2\u003e\u003cp\u003eThe Verogen ForenSeq Imagen Kit generates dual-indexed libraries for sequencing. Each sample is combined with a primer mix that contains a pair of tagged oligos for each target sequence. PCR cycles link the tags to copies of each target, forming DNA templates consisting of the regions of interest flanked by universal primer binding sequences. The tags are then used to attach index adapters and the resulting library is amplified, purified, and pooled for sequencing.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eThe table provides a summary of the number of markers associated with both primer sets in the Verogen ForenSeq Imagen Kit.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSNP category\u003c\/th\u003e\n\u003cth\u003eNumber of markers\u003c\/th\u003e\n\u003cth\u003eAmplicon size range\u003cbr\u003e(bp)\u003c\/th\u003e\n\u003cth colspan=\"2\"\u003eIncluded in DNA primer\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003eMix E\u003c\/td\u003e\n\u003ctd\u003eMix F\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eY-SNPs*\u003c\/td\u003e\n\u003ctd\u003e14\u003c\/td\u003e\n\u003ctd\u003e88–130\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhenotypic SNPs\u003c\/td\u003e\n\u003ctd\u003e41\u003c\/td\u003e\n\u003ctd\u003e69–227\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHair color\u003c\/td\u003e\n\u003ctd\u003e22\u003c\/td\u003e\n\u003ctd\u003e92–227\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEye color\u003c\/td\u003e\n\u003ctd\u003e6\u003c\/td\u003e\n\u003ctd\u003e73–119\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkin color\u003c\/td\u003e\n\u003ctd\u003e36\u003c\/td\u003e\n\u003ctd\u003e69–213\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiogeographical** ancestry SNPs\u003c\/td\u003e\n\u003ctd\u003e56\u003c\/td\u003e\n\u003ctd\u003e67–200\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* 4 SNPs overlap across eye, skin and hair color phenotypes; 13 SNPs overlap across hair and skin color phenotypes; and 2 SNPs overlap across eye and skin color phenotypes.\u003cbr\u003e** Only available in the EVC+BGA SNP panel (DPMF-Geo).\u003c\/p\u003e\u003ch2\u003eProcedure\u003c\/h2\u003e\u003cp\u003eThe ForenSeq Imagen workflow, comprising the ForenSeq Imagen kit, the MiSeq FGx Sequencing System with the MiSeq FGx Reagent Micro kit, and the ForenSeq Imagen analysis methods in the Universal Analysis Software (UAS), is a fully validated DNA-to-data workflow specifically designed for forensic genomics applications (see figure Verogen ForenSeq Imagen Kit workflow). \u003c\/p\u003e\n\u003cp\u003ePrepare the ForenSeq Imagen assay with DPME for External Visible Characteristics (EVC) and Appearance SNPs or DPMF (Geo) for EVC and Biogeographical Ancestry (BGA) SNPs.  \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eSequence with the MiSeq FGx Sequencing System using the MiSeq FGx Reagent Micro Kit.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eAnalyze with dedicated analysis modules preconfigured for Imagen and Imagen Geo analysis methods.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eBatch-download multiple report types from Imagen and Imagen Geo. Phenotype analysis reports are compatible with the HIrisPlex-S web application.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eUpload the phenotype report to the HIrisPlex-S web application for estimations.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eGenerate your reports. The data is reformatted in accessible form with the I-HAT macro tool hosted on the Verogen Data Analysis Server.\u003c\/p\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\u003cp\u003eForensic DNA Phenotyping (FDP) can predict externally visible characteristics from DNA traces at a crime scene. FDP analysis can help focus police investigations with information on an unknown perpetrator when standard DNA profiling fails to inform on identification. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eThe simple Verogen ForenSeq Imagen Kit plate-based assay is part of an integrated FDP workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Seamless report integration with 3rd party software such as HIrisPlex-S allows users to access enhanced lead generation insights.\u003c\/p\u003e\u003ch2\u003eSoftware\u003c\/h2\u003e\u003cp\u003eData generated by the ForenSeq Imagen kit is optimized for analysis using the Universal Analysis Software. Two fit-for-purpose analysis methods enable labs to analyze data from the DPME or DPMF (Geo) primer mixes separately. The UAS generates SNP reports that can be uploaded to the HIrisPlex-S web application for predictions powered by solutions described in multiple peer-reviewed publications. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eTo allow easy interpretation of complex statistical information, reports from the HIrisPlex-S web application can be parsed by the Imagen-HIrisPlex-S Analysis Tool (I-HAT) application on the UAS data server. The I-HAT macro generates human-readable reports that summarize the predictions and probabilities associated with typed SNPs. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eAutomation of the lab workflow allows labs to onboard the entire ForenSeq Imagen workflow from lab to report with confidence. Robust out-of-the-box thresholds and settings allow easy validation and implementation. Rich data visualization capabilities enable labs to quickly gauge the quality of their sequencing run and SNP calls. Extensive filtering and sorting capabilities enable manual review of markers that require inspection.\u003c\/p\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/validationandtechnicaldocument\/qpro-6234-ti-hid-forenseq-imagenkit-1224-ww\" target=\"_blank\" rel=\"noopener\"\u003eForenSeq Imagen Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/validationandtechnicaldocument\/forenseq-imagen-materials-epcr1-vd2022012-a\" target=\"_blank\" rel=\"noopener\"\u003eForenSeq Imagen Kit with the ForenSeq Enhanced PCR1 Buffer System\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/kithandbookandprotocol\/hb-3614-001-hb\" target=\"_blank\" rel=\"noopener\"\u003eForenSeq Imagen Kit Handbook for PrepStation\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/kithandbookandprotocol\/forenseq-imagen-reference-guide-pcr1-vd2022008-a\" target=\"_blank\" rel=\"noopener\"\u003eForenSeq Imagen Kit Reference Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/kithandbookandprotocol\/forenseq-imagen-reference-guide-epcr1-vd2022011-a\" target=\"_blank\" rel=\"noopener\"\u003eForenSeq Imagen Kit with the Enhanced PCR1 Buffer System Reference Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/analysissoftware\/universal-analysis-software-v2-reference-guide-imagen-vd2022014-reva\" target=\"_blank\" rel=\"noopener\"\u003eUniversal Analysis Software Imagen Module V2 Reference Guide # VD2022014 Rev. A August 2022\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/brochureandguide\/prom-19028-001-1125732-fly-hid-ngs-0821-ww\" target=\"_blank\" rel=\"noopener\"\u003eA new horizon of human identification possibilities with NGS\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.qiagen.com\/us\/resources\/brochureandguide\/qpro-11254-bro-hid-ngs-in-hid-0825-ww\" target=\"_blank\" rel=\"noopener\"\u003eReimagining Human Identification\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. no.\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003eV16000189\u003c\/td\u003e\n\u003ctd\u003eVerogen ForenSeq Imagen Kit\u003c\/td\u003e\n\u003ctd\u003eIncludes the required reagents to process up to 96 samples simultaneously for phenotypic and biogeographical ancestry information to generate comprehensive DNA profiles\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\n\u003c\/table\u003e\u003cp\u003e\u003cem\u003eThe Verogen ForenSeq Imagen Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.\u003c\/em\u003e\u003c\/p\u003e","brand":"QIAGEN","offers":[{"title":"Default Title","offer_id":46798469464143,"sku":"V16000189","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/1_5_WebFullSizeImage_7fc8a1c7-e14d-4ce8-aa2c-bdcde88c71c1.jpg?v=1789653406","url":"https:\/\/www.danyel.co.il\/products\/qiagen-forenseq-imagen-kit-v16000189","provider":"danyel biotech","version":"1.0","type":"link"}