{"product_id":"prp-3-12-20-um-21-2-x-250-mm","title":"PRP-3 12-20 µm, 21.2 x 250 mm","description":"\u003cp\u003eHamilton \u003ca href=\"https:\/\/www.hamiltoncompany.com\/hplc-columns\/reversed-phase\/polymer-packings\/prp-3\/prp-3-columns\"\u003ePRP-3 columns\u003c\/a\u003e are based on the PRP-1 with a larger pore size for the separation of macromolecules such as DNA, RNA oligos, proteins, peptides, and proteomics. This column resin has no silanol groups, so irreversible protein adsorption is not a concern, giving analytes such as proteins a recovery of \u0026gt; 90%. This stable material also allows the use of concentrated acids, chaotropes, and even detergents to minimize problems with protein solubility.\u003cbr\u003e\u003cbr\u003eApplication Examples: \u003ca href=\"https:\/\/www.hamiltoncompany.com\/hplc-applications\/packing-material?packingMaterial=PRP-3\"\u003eOrganic compounds: large molecules (\u0026gt; 2,000 mw), peptides, proteins, protein digests, protected and deprotected oligonucleotides, nucleic acids\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSeparation Mode\u003c\/th\u003e\n\u003ctd\u003eReversed-Phase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStationary Phase\u003c\/th\u003e\n\u003ctd\u003ePRP-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eHPLC Column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHardware Inner Diameter\u003c\/th\u003e\n\u003ctd\u003e21.2 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHardware Length\u003c\/th\u003e\n\u003ctd\u003e250 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eParticle Size\u003c\/th\u003e\n\u003ctd\u003e12-20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePacking Material Type\u003c\/th\u003e\n\u003ctd\u003ePSDVB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePore Size\u003c\/th\u003e\n\u003ctd\u003e300 Å\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eParticle Surface Area (m2\/g)\u003c\/th\u003e\n\u003ctd\u003e48\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePore Volume (cm3\/g)\u003c\/th\u003e\n\u003ctd\u003e0.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHardware Material\u003c\/th\u003e\n\u003ctd\u003eStainless Steel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMax Pressure\u003c\/th\u003e\n\u003ctd\u003e5,000 psi\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMobile Phase Limits\u003c\/th\u003e\n\u003ctd\u003epH 1-13, 0-100% aqueous, organic modifier\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Strength\u003c\/th\u003e\n\u003ctd\u003e0.0-0.5N\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemp Limits\u003c\/th\u003e\n\u003ctd\u003e5-85°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRestoration\u003c\/th\u003e\n\u003ctd\u003eRun a gradient of 100% water to 100% acetonitrile. Repeat three times\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompound Classification\u003c\/th\u003e\n\u003ctd\u003ePeptides\/Proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUOM\u003c\/th\u003e\n\u003ctd\u003e1 EACH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hamiltoncompany.com\/knowledge-base\/sds\/prp-3-sds\"\u003ePrp 3 Sds (Sds)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hamiltoncompany.com\/knowledge-base\/application-note\/gpcrs-the-biological-traffic-modulator\"\u003eGpcrs The Biological Traffic Modulator (Application Note)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hamiltoncompany.com\/product-specs\/79469\"\u003e79469 (Specification)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Hamilton Company","offers":[{"title":"Default Title","offer_id":46487127588943,"sku":"79469","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/PRP3_2025-05-19-215552_fjia.jpg?v=1787651289","url":"https:\/\/www.danyel.co.il\/products\/prp-3-12-20-um-21-2-x-250-mm","provider":"danyel biotech","version":"1.0","type":"link"}