{"product_id":"protein-purification-with-hitrap-talon-crude-columns-29048565","title":"Protein purification with HiTrap™ TALON crude columns","description":"\u003cp\u003eHiTrap TALON® crude 1 mL and 5 mL columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eColumns prepacked with cobalt-based chromatography resins designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).\u003c\/li\u003e\n\u003cli\u003eSuitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.\u003c\/li\u003e\n\u003cli\u003eCompatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.\u003c\/li\u003e\n\u003cli\u003ePrepacked HiTrap TALON® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.\u003c\/li\u003e\n\u003cli\u003eHiTrap TALON® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eTALON® Superflow™ is a cobalt-based IMAC resin offering different selectivity compared to nickel-charged resins. TALON® Superflow™ and prepacked HiTrap TALON® crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.\u003c\/p\u003e\u003cp\u003eHiTrap TALON® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the TALON® Superflow™ beads. Prior to loading samples on HiTrap TALON® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze\/thaw.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16076-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16063-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 1 mL","offer_id":45982381441103,"sku":"29048565","price":298.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 1 mL","offer_id":45982381473871,"sku":"28953766","price":1156.0,"currency_code":"ILS","in_stock":true},{"title":"5 × 5 mL","offer_id":45982381506639,"sku":"28953767","price":4174.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-3718-original-png_5defe1ec-0a79-4e8a-81e0-c71bfdceb9e4.png?v=1785960732","url":"https:\/\/www.danyel.co.il\/products\/protein-purification-with-hitrap-talon-crude-columns-29048565","provider":"danyel biotech","version":"1.0","type":"link"}