{"product_id":"probequant-g-50-micro-columns-28903408","title":"ProbeQuant G-50 Micro Columns","description":"\u003cp\u003eProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for the preparation of DNA probes, separating them from unincorporated labeled nucleotides.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor rapid purification of labeled DNA (≥ 20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μL using spin-column chromatography\u003c\/li\u003e\n\u003cli\u003ePrepacked with Sephadex G-50 DNA Grade and pre-equilibrated in STE containing 0.15% Kathon CG\/ICP Biocide for convenience\u003c\/li\u003e\n\u003cli\u003eReady to use and requires less than 4 minutes from sample application to collection of purified product\u003c\/li\u003e\n\u003cli\u003eDesigned for use in a microcentrifuge\u003c\/li\u003e\n\u003cli\u003eTested in assays: nickase, single and double-stranded exonuclease and RNase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation.\u003c\/p\u003e\n\n\u003cp\u003eThe columns are designed for use in a microcentrifuge, come prepacked with Sephadex G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μL to 50 μL.\u003c\/p\u003e\n\n\u003cp\u003eThe resin used in these columns, \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-50-dna-grade-p-00113\"\u003eSephadex G-50 DNA Grade\u003c\/a\u003e, can be used in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003c\/p\u003e\n\n\u003cp\u003e\u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/microspin-columns-p-00044\"\u003eMicroSpin columns\u003c\/a\u003e packed with Sephadex G-50 DNA Grade are ideal for the rapid purification of DNA for use in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides .\u003c\/p\u003e\n\n\u003cp\u003eSephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established size exclusion chromatography resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eAlternatively, \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-25-dna-grade-p-00054\"\u003eSephadex G-25\u003c\/a\u003e or \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-100-dna-grade-p-00198\"\u003eSephadex G-100\u003c\/a\u003e can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eSephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.\u003c\/p\u003e\n\n\u003cp\u003eAll these Sephadex types are therefore suitable for the purification of oligonucleotides or small DNA fragments following synthesis or a labeling reaction.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28903408PL.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"50 columns","offer_id":45982269734991,"sku":"28903408","price":1501.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-8670-original-png_2d100340-4687-42b5-8e93-972d4a141f9b.png?v=1785959651","url":"https:\/\/www.danyel.co.il\/products\/probequant-g-50-micro-columns-28903408","provider":"danyel biotech","version":"1.0","type":"link"}