{"product_id":"prisma-elisa-kit-29707299","title":"PrismA ELISA™ kit","description":"\u003cp\u003eThe PrismA ELISA™ kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA™ chromatography resin and Fibro™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMatched PrismA protein A ligand included in the kit removes the need to source the standard separately and create a custom protocol\u003c\/li\u003e\n\u003cli\u003eParts per billion sensitivity minimizes the risk of undetected residual PrismA ligand\u003c\/li\u003e\n\u003cli\u003eHigh IgG tolerance simplifies sample preparation\u003c\/li\u003e\n\u003cli\u003eConsistently low intra- and inter-plate variability ensures reproducible data\u003c\/li\u003e\n\u003cli\u003eAll-in-one kit containing all reagents needed to perform the assay\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe PrismA ELISA™ kit contains all reagents required to perform the assay. This includes the PrismA ligand optimized for use in ELISA and polyclonal antibodies raised specifically against the PrismA ligand. The PrismA ELISA™ kit was developed with a focus on usability, robustness, and environmental sustainability.\u003c\/p\u003e\n\n\u003cp\u003eHigh IgG tolerance\u003c\/p\u003e\n\n\u003cp\u003eDownstream samples generally have high concentrations of IgG, which creates a challenge for protein A measurement. Accurate measurement requires dissociation of the ligand and IgG, which is especially tricky when the concentration of protein A ligand is very low (\u0026lt; 1 ppm) due to the large excess of IgG.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"PrismA ELISA kit IgG tolerance (Recovery)\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/vOMcoexxmUehXuexF6aS7Q-webp\" style=\"width: 520px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eTo determine the effect of IgG on dissociation, we spiked high, medium, and low concentrations of PrismA ligand into decreasing concentrations of IgG. The PrismA ELISA™ kit could accurately determine PrismA concentration in samples containing as high as 20 mg\/mL of IgG.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eThe PrismA ELISA™ kit\u003c\/strong\u003e delivers high sensitivity for detecting trace amounts of ligand—even during the final stages of purification:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003e\n\u003cstrong\u003eLower limit of detection (LLD):\u003c\/strong\u003e 10 pg\/mL (+IgG)\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cstrong\u003eLower limit of quantification (LLQ):\u003c\/strong\u003e 10 pg\/mL (+IgG)\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/ZrSLDTPBG02bQjAy7hYx4g-pdf\"\u003eView data file\u003c\/a\u003e to see supporting data for these performance specifications.\u003c\/p\u003e\n\n\u003cp\u003ePerform with confidence across common buffer conditions\u003c\/p\u003e\n\n\u003cp\u003eThe PrismA ELISA™ kit delivers accurate and reproducible results over multiple experiments, and has been tested with common buffers used in downstream purification of monoclonal antibodies. The results showed the kit was compatible with all five buffers tested, as indicated by all dilutions of each sample (8\/8) being within the acceptance criteria of the assay, and the parallelism being below the target of 20%.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"PrismA ELISA™ kit buffer compatibility\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/kpSkRzVzJEym9-YYVvJ5_w-webp\" style=\"width: 520px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eContents of the PrismA ELISA™ kit include:\u003c\/p\u003e\n\n\u003ctable class=\"table table-striped\"\u003e\n\t\u003cthead\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003cth\u003eComponent\u003c\/th\u003e\n\t\t\t\u003cth\u003eVolume\u003c\/th\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/thead\u003e\n\t\u003ctbody\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Assay Buffer (1X)\u003c\/td\u003e\n\t\t\t\u003ctd\u003e10 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Detection Antibody Diluent (1X)\u003c\/td\u003e\n\t\t\t\u003ctd\u003e15 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Wash Buffer (10X)\u003c\/td\u003e\n\t\t\t\u003ctd\u003e25 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit TMB (1X)\u003c\/td\u003e\n\t\t\t\u003ctd\u003e15 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Dissociation Buffer (1X)\u003c\/td\u003e\n\t\t\t\u003ctd\u003e30 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Stop Buffer (1X)\u003c\/td\u003e\n\t\t\t\u003ctd\u003e15 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Standard\u003c\/td\u003e\n\t\t\t\u003ctd\u003e1 vial\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Detection Antibody\u003c\/td\u003e\n\t\t\t\u003ctd\u003e140 µL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit 96 Well Strip-Plate\u003c\/td\u003e\n\t\t\t\u003ctd\u003e1 plate\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd\u003ePrismA ELISA™ Kit Plate Seal\u003c\/td\u003e\n\t\t\t\u003ctd\u003e1 seal\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/ZrSLDTPBG02bQjAy7hYx4g-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29707991.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"96 well plate \/ 1 unit","offer_id":45982278647887,"sku":"29707299","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/bDu2w60pDUWyjAri75Bjxg-original-png_15011a6b-933b-4062-9d43-a04ca02c4f7f.png?v=1785959792","url":"https:\/\/www.danyel.co.il\/products\/prisma-elisa-kit-29707299","provider":"danyel biotech","version":"1.0","type":"link"}