{"product_id":"poly-a-polymerase-tailing-kit-pap5104h","title":"Poly(A) Polymerase Tailing Kit","description":"\u003cp\u003ePoly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3´-hydroxyl termini of RNA molecules. 6 The Poly(A) Polymerase Tailing Kit provides the enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3´ end of any RNA. Poly(A) Polymerase is encoded by an E. coli gene that has been cloned into a plasmid and over-expressed in an E. coli strain.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eAddition of a poly(A) tail to RNA synthesised in vitro in order to increase the stability of the RNA and enhance its ability to be translated in vivo after transfection or microinjection into eukaryotic cells. 1-3\u003cbr\u003eAddition of a poly(A) tail to an RNA molecule or a mixture of RNA molecules in order to provide a priming site for synthesis of first-strand cDNA using a primer with poly(dT) on its 3´ end.\u003cbr\u003eSynthesis of polyadenylated RNA for nucleic acid amplification methods or gene expression studies.\u003cbr\u003e3´-end labelling of RNA with radioactive A residues. 4\u003cbr\u003eQuantifying mRNA. 5\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eDrummond, D.R. et al. (1985) J. Cell. Biol. 100, 1148.\u003cbr\u003eGalili, G. et al. (1988) J. Biol. Chem. 263, 5764.\u003cbr\u003eBelasco, J. and Brawerman, G. (1993) Control of Messenger RNA Stability, Academic Press, San Diego, CA.\u003cbr\u003eLingner, J. and Keller, W. (1993) Nucleic Acids Res. 21, 2917.\u003cbr\u003eKrug, M.S. and Berger, S.L. (1987) Methods Enzymol. 152, 262.\u003cbr\u003eGething, M. et al. (1980) Nature 287, 301.\u003c\/p\u003e\u003cp\u003eFigure 1. Poly(A) tailing of RNA with the Poly(A) Polymerase Tailing Kit. The entire final product of an AmpliCap-Max™ in vitro transcription capping reaction (60 µg of a 1,760-base luciferase RNA) was treated with 8 units of Poly(A) Polymerase for the indicated times. For each sample, 0.1 µg aliquots were analysed on a 1% agarose-formaldehyde gel.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePolyadenylate 3' ends of RNA molecules for various downstream applications\u003c\/li\u003e\n\u003cli\u003eQuality Kit: High enzymatic purity, specificity, and activity\u003c\/li\u003e\n\u003cli\u003eFlexible: Use polyadenylated RNAs in a variety of applications such as stabilising in vitro transcribed RNA and adding primer (oligo-dT) binding sites to any RNA for first-strand cDNA synthesis\u003c\/li\u003e\n\u003cli\u003eFast and Easy: Simple protocol produces polyadenylated RNA molecules quickly\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of Poly(A) Polymerase catalyses the incorporation of 1 nmol of AMP into acid-insoluble form in 10 minutes at 37 °C under standard assay conditions.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003e10X Reaction Buffer: 0.5 M Tris-HCl (pH 8.0), 2.5 M NaCl, and 100 mM MgCl2. A separate 10-mM ATP Solution is also provided.\u003c\/p\u003e\u003cp\u003eQuality Control: Poly(A) Polymerase is tested for polyadenylation of RNA in vitro. It is free of detectable exo- and endonuclease and RNase activity.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContents: Poly(A) Polymerase, 10X Reaction Buffer, 10 mM ATP, Sterile RNase-Free Water.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_PAP5104H_Poly-A-Polymerase-Tailing-Kit\"\u003ePoly A Polymerase Tailing Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PAP5104H_PolyA-Polymerase\"\u003ePolyA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PAP5104H_poly(a)-polymerase-tailing-kit\"\u003epoly(a) polymerase tailing kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PAP5104H_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986472263759,"sku":"PAP5104H","price":1618.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Poly_2528A_2529-Polymerase-Tailing-Kit_4a6d6c7c-daf8-4e5f-8c43-4ee4bb29b9ce.jpg?v=1785956763","url":"https:\/\/www.danyel.co.il\/products\/poly-a-polymerase-tailing-kit-pap5104h","provider":"danyel biotech","version":"1.0","type":"link"}