{"product_id":"plurispin-human-nk-cell-depletion-pluriselect","title":"pluriSpin Human NK Cell Depletion","description":"\u003cp\u003epluriSpin human NK-cell depletion, for negative selection of cells, has been developed to isolate a clean, vital and untouched NK-cell free cell population from whole blood, buffy coat, cord blood, bone marrow or similar sample materials. The product labels all NK-cells and leaves the NK-cell free fraction untouched.\u003c\/p\u003e\u003cp\u003eSubsequent PBMC spin density gradient centrifugation is used to remove all labeled cells from the sample together with the red blood cells (RBCs) and granulocytes. The untouched and unlabeled NK-cell free fraction remains enriched in the interphase between the plasma and the density medium. We recommend PBMC Spin Medium as the density medium for DGM centrifugation. Alternatively, Ficoll, Lymphoprep or other comparable density media can be used without changing the protocol.\u003c\/p\u003e\u003ch2\u003eFeatures\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNegative selection\u003c\/strong\u003e – Unwanted cells are labeled with specific antibodies and then removed, while the desired cells remain unlabeled in the suspension and retain their natural properties.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh purity and cell viability\u003c\/strong\u003e – The isolated cells remain functional and can be used directly for subsequent experiments such as flow cytometry (FACS), cell culture or molecular biological analyses.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNo magnetic beads orcolumnsrequired\u003c\/strong\u003e – Unlike many other cell separation methods, pluriSpin does not require magnetic beads or columns, which simplifies the process and preserves cell integrity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible\u003c\/strong\u003e – Purification by density centrifugation can be carried out using PBMC Spin Medium or comparable density media from other manufacturers, such as Lymphopreo, Ficoll or Pancoll,\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell type\u003c\/th\u003e\n\u003ctd\u003eNK cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eCD56\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt; 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation platform\u003c\/th\u003e\n\u003ctd\u003enon-magnetic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation method\u003c\/th\u003e\n\u003ctd\u003enegative, depletion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample material\u003c\/th\u003e\n\u003ctd\u003ewhole blood, PBMC, Buffy Coat, cord blood, bone marrow, primary cell suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCan be combined with\u003c\/th\u003e\n\u003ctd\u003ePBMC Spin Medium, pluriMate separation tubes, Lymphoprep™, Ficoll™, Pancoll, SepMate™-15 ml tubes, SepMate™-50 ml tubes, Leucosep 12 ml tubes, Leucosep 50 ml tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTerms of delivery\u003c\/th\u003e\n\u003ctd\u003eambient temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage condition\u003c\/th\u003e\n\u003ctd\u003e4 - 8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRegulatory statement\u003c\/th\u003e\n\u003ctd\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget cells\u003c\/th\u003e\n\u003ctd\u003enatural killer cells (NK cells)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell depletion\u003c\/th\u003e\n\u003ctd\u003ePerformed with PBMC Spin density gradient medium.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompatible with\u003c\/th\u003e\n\u003ctd\u003ePBMC 24+ Spin Medium, Lymphoprep, Ficoll, Pancoll\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. No.\u003c\/th\u003e\n\u003cth\u003ePacking\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e19-05002-31\u003c\/td\u003e\n\u003ctd\u003e1 vial (2 ml)\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"pluriSelect","offers":[{"title":"Default Title","offer_id":68092780511311,"sku":"19-05002-31","price":1090.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-product-1247.png?v=1794313217","url":"https:\/\/www.danyel.co.il\/products\/plurispin-human-nk-cell-depletion-pluriselect","provider":"danyel biotech","version":"1.0","type":"link"}