{"product_id":"pluribead-murin-cd4-cell-separation-pluriselect","title":"pluriBead Mouse CD4 Cell Separation","description":"\u003cp\u003eCD4 pluriBeads are suitable for rapid, positive cell separation of CD4+ cells from murine sample material. The target-specific non-magnetic pluriBeads can be used without sample preparation for cell isolation, directly in blood (whole blood), buffy coat or cell cultures. For mixing the CD4-specific pluriBeads with the sample material, we recommend our universal mixing device the pluriPlix or a horizontal mixer.\u003c\/p\u003e\u003cp\u003eDuring the incubation period, the CD4+ cells from the sample bind to the specific surface of the pluriBeads. The pluriBeads, with the bound cells, are then separated from all unbound cells using a pluriStrainer. In the final step, the target cells are gently detached from the pluriBeads using our detachment buffer.\u003c\/p\u003e\u003cp\u003eThe enriched, particle-free CD4+ cells are immediately available for further downstream applications such as cell culture experiments or FACS analyses. For the isolation of cell-specific RNA, DNA or proteins, the bound cells can be lysed directly on the pluriStrainer without the detachment step.\u003c\/p\u003e\u003ch2\u003eBead Size\u003c\/h2\u003e\u003cp\u003epluriBeads are available as S-pluriBead and M-pluriBead; S and M define the size of the microbeads.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eS-pluriBead\u003c\/strong\u003e – 32 µm in diameter, for a small number of target cells (\u0026lt; 1 × 10^6 cells \/ ml sample volume)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eM-pluriBead\u003c\/strong\u003e – 65 µm in diameter, for a high number of target cells (\u0026gt; 1 × 10^6 cells \/ ml sample volume)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eFeatures\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNo sample preparation\u003c\/strong\u003e – Use 200 µl - 45 ml whole blood sample material without prior density gradient centrifugation or red blood cell lysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast, gentle cell isolation\u003c\/strong\u003e – Isolate cells in just 5 minutes (depending on the cell type and the amount of target cells available).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimultaneous cellisolation\u003c\/strong\u003e – Combine S- and M-pluriBeads and isolate two different cell types from one sample at the same time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSequential cell isolation\u003c\/strong\u003e – Unbound cells in a sample are not lost, but can be concentrated and reused for isolation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eMurin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eCD4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell type\u003c\/th\u003e\n\u003ctd\u003eMonocyte subpopulations, Helper T cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation method\u003c\/th\u003e\n\u003ctd\u003epositive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell separation platform\u003c\/th\u003e\n\u003ctd\u003enon-magnetic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample material\u003c\/th\u003e\n\u003ctd\u003ewhole blood, PBMC, Buffy Coat, cord blood, bone marrow, primary cell suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTerms of delivery\u003c\/th\u003e\n\u003ctd\u003eambient temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage condition\u003c\/th\u003e\n\u003ctd\u003e4 - 8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRegulatory statement\u003c\/th\u003e\n\u003ctd\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Cells\u003c\/th\u003e\n\u003ctd\u003eCD4+ Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Types\u003c\/th\u003e\n\u003ctd\u003eMonocyte subpopulation, helper T cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Enrichment\u003c\/th\u003e\n\u003ctd\u003ewith 30 or 60 µm pluriStrainer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetachability\u003c\/th\u003e\n\u003ctd\u003eYes, the CD4+ cells can be detached from the pluriBead.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eOrdering Information\u003c\/h2\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCat. No.\u003c\/th\u003e\n\u003cth\u003eBead size\u003c\/th\u003e\n\u003cth\u003ePacking\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e29-00400-10\u003c\/td\u003e\n\u003ctd\u003eS-pluriBead (32 µm)\u003c\/td\u003e\n\u003ctd\u003e1 vial (2 ml)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e29-00400-20\u003c\/td\u003e\n\u003ctd\u003eM-pluriBead (65 µm)\u003c\/td\u003e\n\u003ctd\u003e1 vial (2 ml)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"pluriSelect","offers":[{"title":"S-pluriBead (32 µm)","offer_id":68092784345167,"sku":"29-00400-10","price":517.0,"currency_code":"ILS","in_stock":true},{"title":"M-pluriBead (65 µm)","offer_id":68092784377935,"sku":"29-00400-20","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/pluriselect-product-1245.png?v=1794313217","url":"https:\/\/www.danyel.co.il\/products\/pluribead-murin-cd4-cell-separation-pluriselect","provider":"danyel biotech","version":"1.0","type":"link"}