{"product_id":"plasmid-safe-atp-dependent-dnase-mbenz-002","title":"Plasmid-Safe ATP-Dependent DNase","description":"\u003cp\u003ePlasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations. Such preparations are frequently contaminated with fragments of bacterial genomic DNA generated during alkaline lysis. Other purification options, such as spin-columns or even CsCl centrifugation, do not effectively remove these contaminants and require further purification steps. Contaminating DNA fragments left behind by these methods ultimately can become ligated into a cloning vector, resulting in false positives and high backgrounds, or erroneous sequence data.\u003c\/p\u003e\u003cp\u003ePlasmid-Safe ATP-Dependent DNase digests linear dsDNA to deoxynucleotides at slightly alkaline pH and, with lower efficiency, closed-circular and linear ssDNA. The enzyme has no activity on nicked or closed-circular dsDNA or supercoiled DNA. Therefore, Plasmid-Safe DNase is ideal as the final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations.\u003c\/p\u003e\u003cp\u003eFigure 1. Plasmid-Safe™ ATP-Dependent DNase removes contaminating genomic DNA from plasmid preps. Lane 1, 3 µg of Sma I-digested bacterial chromosomal DNA; lane 2, 500 ng of uncut plasmid DNA; lane 3, mixture of 3 µg of digested bacterial chromosomal DNA and 500 ng of uncut plasmid before Plasmid-Safe DNase treatment; lane 4, mixture of chromosomal DNA and plasmid DNA after Plasmid-Safe DNase treatment (incubation with Plasmid-Safe DNase for 30 minutes at 37 °C); lane M, Kilobase ladder.\u003c\/p\u003e\u003cp\u003eBenefits\u003c\/p\u003e\u003cp\u003eMinimises the possibility of cloning or sequencing contaminating chromosomal DNA from plasmid, cosmid, fosmid, or BAC preparations.\u003cbr\u003eFast and easy protocol with minimal handling time.\u003cbr\u003eComplete protocols provided for using Plasmid-Safe DNase with miniprep, midiprep, and maxiprep plasmid, cosmid, fosmid, and BAC DNA purifications.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRemove contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC preps\u003c\/li\u003e\n\u003cli\u003eDigest contaminating linear DNA without digesting nicked or closed-circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.)\u003c\/li\u003e\n\u003cli\u003eUse as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations\u003c\/li\u003e\n\u003cli\u003eUtilise whenever protecting circular dsDNA is important\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of Plasmid-Safe DNase converts 1 nmol of deoxynucleotides in linear T7 DNA into an acid-soluble form in 30 minutes at 37 °C under standard assay conditions. Three units will digest 1 µg of DNA in 30 minutes at 37 °C.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003ePlasmid-Safe 10X Reaction Buffer: 330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium acetate, and 5.0 mM DTT. ATP must be added to a final concentration of 1 mM in the 1X Buffer.\u003c\/p\u003e\u003cp\u003eQuality Control: Plasmid-Safe DNase is free of detectable RNase and double-stranded, DNA-specific endonuclease activities.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eIncludes Plasmid-Safe 10X Reaction Buffer and 25 mM ATP Solution.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_MBENZ-002_E3101K-E3110K-Plasmid-Safe-ATP-Dependent-DNase\"\u003eE3101K E3110K Plasmid Safe ATP Dependent DNase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_MBENZ-002_PlasmidSafe-DNase\"\u003ePlasmidSafe DNase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_MBENZ-002_plasmid-safe-atp-dependent-dnase\"\u003eplasmid safe atp dependent dnase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_MBENZ-002_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 U\/µL, 1,000 Units","offer_id":45986440675407,"sku":"E3101K","price":653.0,"currency_code":"ILS","in_stock":true},{"title":"10 U\/µL, 10,000 Units","offer_id":45986440708175,"sku":"E3110K","price":3337.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Plasmid-Safe-ATP-Dependent-DNase_cf0c2145-7009-43a3-ba07-aedf4dbc9f20.jpg?v=1785956233","url":"https:\/\/www.danyel.co.il\/products\/plasmid-safe-atp-dependent-dnase-mbenz-002","provider":"danyel biotech","version":"1.0","type":"link"}